IOVS News in Physiological Sciences
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 1971;10:41-53.)
© 1971 by The Association for Research in Vision and Ophthalmology, Inc.

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by ANDERSON, E. I.
Right arrow Articles by SPECTOR, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by ANDERSON, E. I.
Right arrow Articles by SPECTOR, A.

Oxidation-Reduction Reactions Involving Ascorbic Acid and the Hexosemonophosphate Shunt in Corneal Epithelium

E. I. ANDERSON 1 and ABRAHAM SPECTOR 1

1 Department of Ophthalmology, College of Physicians and Surgeons, Columbia University, New York, N. Y.

A dehydroascorbic acid reductase and a glutathione peroxidase have been shown to be present in corneal epithelium. Ascorbic acid (ASC), dehydroascorbate (DHA), or hydrogen peroxide (H2O2) can be coupled to the glutathione reductase system to effect nicotinamide adenine dinucleotide phosphate (NADPH) oxidation. A coupling of the hexosemonophosphate (HMP) shunt via the pyridine nucleotide, NADPH, to DHA has been demonstrated. The suggestion is made that the rate of ASC oxidation in vivo is probably adequate to reoxidize a significant fraction of the NADPH generated by the shunt. ASC, glutathione (GSH), H2O2, and total soluble thiol concentrations of corneal epithelium were determined. At levels of H2O2 within the observed physiological limit, endogenous catalase is inactive.

Key Words: dehydroascorbic acid reductase • glutathione peroxidase • oxidation-reduction • corneal epithelium • ascorbic acid • glutathione • hexosemonophosphate shunt

Submitted on November 9, 1970
Accepted on November 30, 1970






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1971 by the Association for Research in Vision and Ophthalmology