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(Investigative Ophthalmology and Visual Science. 1971;10:750-759.)
© 1971 by The Association for Research in Vision and Ophthalmology, Inc.

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Antibody in Tears Following Intranasal Vaccination With Inactivated Virus

II. Enhancement of Tear Antibody Production by the Use of Polyinosinic: Polycytidilic Acid (Poly I:C)

HARRY L. S. KNOPF 1, NEIL R. BLACKLOW 1, MORRIS I. GLASSMAN 1, WALTER L. CLINE 1, and VERNON G. WONG 1

1 Laboratory of Vision Research, National Eye Institute, National Institute of Allergy and Infectious Diseases, and National Cancer Institute, National Institutes of Health, United States Department of Health, Education, and Welfare, Bethesda, Md. 20014

Rabbits were immunized with a formalin-treated vaccinia virus vaccine by means of intranasal or intradermal administration. A comparison was made between animals receiving vaccine with or without the adjuvant poly I:C. Two additional animals received live virus intradermally and were used as controls. The immunoglobulins of the tears and serum were analyzed, and it was found that: (1) IgA (IIS type) was the predominant immunoglobulin in the tears. (2) IgA appeared to be correlated with the antibody activity in tears of intranasally vaccinated animals. (3) The antiviral activity in the tears of intradermally vaccinated animals appeared to be IgG. (4) Poly I:C enhanced both the serum and tear antibody liters. (5) The predominant immunoglobulin and antibody in the serum was IgG. The indirect fluorescent antibody procedure was found to be a useful adjunctive method for assaying tear antibody. The possible mechanisms involved in the induction of tear antibodies and the effects of adjuvants are discussed.

Key Words: tear antibody • poly I:C adjuvant • formalin inactivation • vaccinia virus • intranasal vaccination • fluorescent antibody test

Submitted on July 15, 1971
Accepted on August 3, 1971






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Copyright © 1971 by the Association for Research in Vision and Ophthalmology