IOVS AJP: Gastrointestinal and Liver Physiology
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(Investigative Ophthalmology and Visual Science. 1971;10:100-107.)
© 1971 by The Association for Research in Vision and Ophthalmology, Inc.

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Experimental Rehydration of Cryopreserved Corneal Tissue

HENRY F. EDELHAUSER 1, DIANE L. VAN-HORN 1, ARTHUR B. GALLUN 1, and RICHARD O. SCHULTZ 1

1 Departments of Physiology and Ophthalmology, Medical College of Wisconsin (formerly Marquette School of Medicine), and Wood Veterans Administration Center, Milwaukee, Wis.

Changes in total water and electrolyte content were measured in corneas following cryopreservation and following experimental rehydration of the cryopreserved-thawed tissue in an albumin-NaCl solution, Kinsey medium, or in vivo in aqueous humor. During the cryopreservation procedure the corneal tissue becomes dehydrated and loses intracellular potassium and sodium. Experimental rehydration of the cryopreserved tissue in Kinsey medium restores the Na+ and water content to control values, but corneal K+ remains decreased. Corneas rehydrated in albumin-NaCl regained only the normal water content; whereas, corneas rehydrated in vivo in aqueous humor regained the normal water content and sodium concentration over a two-hour period, while corneal potassium showed a slight increase throughout the two-hour period.

Note:
Read in part before the FASEB Meetings, Atlantic City, N. J., April 16, 1970.

Key Words: cornea, cryopreservation, corneal hydration and electrolyte content • freeze preservation of human and rabbit corneas

Submitted on June 10, 1970
Accepted on December 18, 1970






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Copyright © 1971 by the Association for Research in Vision and Ophthalmology