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(Investigative Ophthalmology and Visual Science. 1972;11:905-915.)
© 1972 by The Association for Research in Vision and Ophthalmology, Inc.

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Calcium-Induced Aggregation of Bovine Lens Alpha Crystallins

J. A. JEDZINIAK 1, J. H. KINOSHITA 2, E. M. YATES 1, L. O. HOCKER 1, and G. B. BENEDEK 1

1 The Department of Physics, Massachusetts Institute of Technology, Cambridge, Mass.
2 The Laboratory of Vision Research, National Eye Institute, National Institute of Health, Bethesda, Md.

The theory of light scattering indicates that aggregation of the crystalline lens proteins can produce opacity. Furthermore, it is known that calcium increases in the cataractous lens. We have, therefore, investigated the effectiveness of calcium ion in producmg aggregates of the soluble proteins of the bovine lens. The experimental results show: (1) CaCl2 produce's aggregates of the soluble protein of the lens; (2) the pre-{alpha} and at fraction of the lens protein are the specific targets of calcium ion even in the presence of sulfhydryl reducing reagent; (3) the aggregation can be reversed and transparency restored when calcium is removed either by dialysis or chelation; and (4) the molecular weight distribution and index of refraction of the aggregates have been measured.

Key Words: light scattering • opacity • calcium • aggregation • pre-{alpha} crystallin • {alpha}4 crystallin • diffusion coefficient • Svedberg constants • index of refraction • reversibility

Submitted on July 1, 1972
Accepted on August 16, 1972




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