IOVS Journal of Applied Physiology
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(Investigative Ophthalmology and Visual Science. 1972;11:127-132.)
© 1972 by The Association for Research in Vision and Ophthalmology, Inc.

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Critical Point Drying of Soft Biological Material for the Scanning Electron Microscope

MORTON E. SMITH 1 and EDWARD H. FINKE 1

1 Departments of Ophthalmology and Pathology, Washington University School of Medicine, St. Louis, Mo. 63110

An apparatus for the dehydration of soft biological material for the scanning electron microscope utilizing the principle of critical point drying is described. The apparatus can be easily and inexpensively constructed from materials available in most scientific laboratories. It is convenient to use and is less time consuming than freeze drying. It produces results which are equal to, or better and indeed more consistent than, other methods of dehydration.

Key Words: scanning electron microscope • critical point drying • freeze drying • freon • air drying • surface tension

Accepted on February 9, 1972






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