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(Investigative Ophthalmology and Visual Science. 1972;11:133-136.)
© 1972 by The Association for Research in Vision and Ophthalmology, Inc.

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Scanning Electron Microscopy Tissue Preparation

DAVID M. WORTHEN 1 and M. GARY WICKHAM 1

1 Research Service, Veterans Administration Hospital, and Department of Ophthalmology, University of Florida, Gainesville, Fla.

Reprint requests to: David M. Worthen, M.D., V. A. Hospital, Gainesville, Fla. 32601.

Cebus monkey angle tissue prepared by either the freeze drying method or the critical point method is superior to that prepared by air drying. The apparatus for the critical point method is less expensive and the technique faster with more consistent if not superior tissue dehydration for scanning electron microscopy.

Key Words: Tissue preparation • scanning electron microscopy • freeze drying • Freon bomb • critical point • Cebus monkey angle

Accepted on February 10, 1972






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