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(Investigative Ophthalmology and Visual Science. 1972;11:662-667.)
© 1972 by The Association for Research in Vision and Ophthalmology, Inc.

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Effect of Lysosomes on Cultured Rabbit Corneal Endothelial Cells

DHARMENDRA V. ARYA 1, JEAN MANNAGH 1, and A. RAY IRVINE Jr. 1

1 Estelle Doheny Eye Foundation Laboratory, Los Angeles, Calif.

Reprint requests: Dr. Dharmendra V. Arya, Estelle Doheny Eye Foundation Laboratory, 272 S.Lake St., Los Angeles, Calif. 90057.

Lysosomal preparations induced marked changes in cultured corneal endothelial cells, firstly causing the epithelial-like pattern of control growth state to become multilayered and mesothelialin appearance and behavior, and secondly causing cell detachment and death. These culture changes were reversible with removal of lysosomes and restoration of fresh growth media. It appears that the changes observed were due to cathepsin, since protease with catheptic activity similar to that oflijsosomal preparations produced a similar effect, whereas the enzymes B-glucuronidase, aryl sulfatase, and acid phosphataseat concentrations found in the lijsosomal preparations did not affect the cells. Trasylal, a mucoprotein protease inhibitor, reduced cell damage, thus suggesting the involvement of a protease or cathepsin in the reaction. A parallel between cathepsin concentrationand the extent of cellular detachment was observed.

Note:
Presented at the Association for Research in Ophthalmology meeting in Florida, 1969.

Key Words: corneal endothelium • tissue culture • lysosomes • cathepsin • cellular detachment

Accepted on May 5, 1972






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