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(Investigative Ophthalmology and Visual Science. 1973;12:771-776.)
© 1973 by The Association for Research in Vision and Ophthalmology, Inc.

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Fibroblast Collagenase Partial Purification and Characterization

RICHARD M. HOOK 1, CAROL WELLER HOOK 1, and STUART I. BROWN 1

1 Cornea Research Laboratory, Department of Ophthalmology, New York Hospital- Cornell University Medical Center, New York, N. Y.

Corneal fibroblasts grown in tissue culture were induced to produce a collagenase when dexamethasone was included in the culture media. This collagenase, when partially purified, was shown to have an approximate molecular weight of 65,000 and was inhibited by cysteine, Na2EDTA, and freshly prepared rabbit serum. The fibroblast control media, i.e., without dexamethasone, contained no detectable collagenase activity. However, when the control media was concentrated and exposed to trypsin or polymorphonuclear leukocyte lysosomal lysates, it showed collagenase activity. This suggested that the fibroblasts produced a pro-collagenase which was activated by trypsin or lysosomal lysates.

Submitted on November 3, 1972
Accepted on July 23, 1973




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G. G. Rose and P. B. Robertson
Collagenolysis by Human Gingival Fibroblast Cell Lines
Journal of Dental Research, April 1, 1977; 56(4): 416 - 424.
[Abstract] [PDF]


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