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(Investigative Ophthalmology and Visual Science. 1973;12:114-126.)
© 1973 by The Association for Research in Vision and Ophthalmology, Inc.

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The Soluble Proteins of the Bovine Cornea

WILLIAM S. HOLT 1 and JIN H. KINOSHITA 1

1 Howe Laboratory of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear Infirmary Boston, Mass.

The soluble proteins of calf cornea, both epithelial and stromal, were fractionated by Sephadex G-200 gel filtration followed by DEAE-cellulose ion-exchange chromatography. Each fraction teas further analyzed using a variety of techniques, including disc electrophoresis in polyacrylamide gels, Ouchterlony immunoprecipitation, and immunoelectrophoresis. From the corneal epithelium some 20 or more distinct protein zones are demonstrated by electrophoresis. A single protein appears to comprise about 40 per cent of the epithelial proteins and a significant part of the stroma as well. The epithelium is devoid of serum proteins. The stroma, however, contains at least five serum proteins, albumin, 7S-gamma globulin, transferrin, a lipoprotein, and an unidentified protein of beta electrophoretic mobility. These serum proteins constitute over half of the soluble stromal proteins. Of the remainder, there is one stromal protein identical with the principal epithelial protein, a few are glycoproteins, and none are soluble collagen

Submitted on July 17, 1972
Accepted on November 6, 1972




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