IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 1973;12:554-566.)
© 1973 by The Association for Research in Vision and Ophthalmology, Inc.

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by TSO, M. O. M.
Right arrow Articles by ZIMMERMAN, L. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by TSO, M. O. M.
Right arrow Articles by ZIMMERMAN, L. E.

Organ Culture of Human Retinal Pigment Epithelium and Choroid: a Model for the Study of Cytologic Behavior of RPE in Vitro

MARK O. M. TSO 1, DANIEL ALBERT 2, and LORENZ E. ZIMMERMAN 1

1 Registry of Ophthalmic Pathology, Armed Forces Institute of Pathology, and the Department of Ophthalmology, The George Washington University Medical Center Washington, D. C.
2 Department of Ophthalmology and Visual Sciences, Yale University School of Medicine New Haven, Conn.

Organ culture of human choroid and retinal pigment epithelium was maintained in medium RPMI 1640 with Millipore filter as support for various periods up to six weeks. The tissues were obtained from autopsy and surgical materials. The cultures were studied by light and electron microscopy. The tissues maintained by this culture method appeared to be in a relatively normal active state, without overt degeneration or proliferation for at least two weeks. The RPE in culture showed capability of phagocytosing particles of thorotrast. Such a culture system serves as a useful model for study of the cytologic behavior of human retinal pigment epithelium (RPE) in vitro, especially during the early stages of disease processes.

Key Words: retinal pigment epithelium • choroid • human • phagocytosis • thorotrast • organ culture

Submitted on February 5, 1973
Accepted on May 1, 1973




This article has been cited by other articles:


Home page
 IOVSHome page
H. J. Hyun, J. H. Sohn, D. W. Ha, Y. H. Ahn, J.-Y. Koh, and Y. H. Yoon
Depletion of Intracellular Zinc and Copper with TPEN Results in Apoptosis of Cultured Human Retinal Pigment Epithelial Cells
Invest. Ophthalmol. Vis. Sci., February 1, 2001; 42(2): 460 - 465.
[Abstract] [Full Text]

Home page
 ScienceHome page
V. Elner, T Schaffner, K Taylor, and S Glagov
Immunophagocytic properties of retinal pigment epithelium cells
Science, January 2, 1981; 211(4477): 74 - 76.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1973 by the Association for Research in Vision and Ophthalmology