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(Investigative Ophthalmology and Visual Science. 1974;13:23-32.)
© 1974 by The Association for Research in Vision and Ophthalmology, Inc.

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Human Corneal Cells in Vitro: Morphology and Histocompatibility (HL-A) Antigens of Pure Cell Populations

DAVID A. NEWSOME 1, MITSUO TAKASUGI 2, KENNETH R. KENYON 1, WALTER F. STARK 3, and GERHARD OPELZ 4

1 Laboratory of Vision Research, National Institutes of Health, United States Department of Health, Education, and Welfare, Bethesda, Md. 20014
2 Department of Surgery, School of Medicine, University of California, Los Angeles, Calif. 90024
3 Clinical Branch, National Eye Institute, National Institutes of Health, United States Department of Health, Education, and Welfare, Bethesda, Md. 20014
4 National Institute of Child Health and Human Development, National Institutes of Health, United States Department of Health, Education, and Welfare, Bethesda, Md. 20014

Pure populations of human comeal epithelial cells, keratocytes, and endothelial cells were established in vitro. Morphologic examination of the cells by phase contrast and electron microscopy showed that, even after multiple serial passages, the cells retained structural specializations typical of their tissues of origin. Using a modified cytotoxic plating inhibition test, HL-A histocompatibility antigens could successfully be detected on all cultured cells. The results of HL-A typing were generally concordant between corneal cells and lymphocytes from the same donor. This study provides direct evidence that HL-A antigens are present on cultured corneal cells, and suggests that such cells may prove useful in the study of immunologic graft rejection encountered in keratoplasty.

Key Words: HL-A histocompatibility antigens • tissue culture • electron microscopy • cornea, human • corneal epithelium • corneal keratocytes • corneal endothelium

Submitted on June 15, 1973




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