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(Investigative Ophthalmology and Visual Science. 1974;13:174-180.)
© 1974 by The Association for Research in Vision and Ophthalmology, Inc.

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The Cornea-Destroying Enzyme of Pseudomonas Aeruginosa

STUART I. BROWN 1, STEPHEN E. BLOOMFIELD 1, and WAI-FONG I. TAM 1

1 Cornea Service and Research Laboratory, New York Hospital-Cornell University Medical Center, 525 E. 68 St., New York, N. Y. 10021

The harvest media from five strains of Pseudomonas aeruginosa collected from severe clinical infections did not contain a collagenase. Instead, an enzyme was isolated that could degrade the ground substance of the cornea. Partial purification of this enzyme indicated that it was the only enzyme in the harvest media that could destroy the rabbit cornea after intralamellar injection. Na2EDTA completely inhibited this enzyme.

Submitted on August 15, 1973
Accepted on October 25, 1973






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