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(Investigative Ophthalmology and Visual Science. 1974;13:575-579.)
© 1974 by The Association for Research in Vision and Ophthalmology, Inc.

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In Vitro Studies of Corneal Wound Healing: Epithelial-Endothelial Interactions

J. DOUGLAS CAMERON 1, B. ALLEN FLAXMAN 2, and MYRON YANOFF 3

1 Department of Ophthalmology and Pathology, Scheie Eye Institute and University of Pennsylvania Medical School, Philadelphia, Pa. 19104
2 Department of Ophthalmology and Pathology, Scheie Eye Institute and University of Pennsylvania Medical School Philadelphia, Pa. 19104
3 Temple University Health Sciences Center, Department of Dermatology, The Skin and Cancer Hospital of Philadelphia, Philadelphia, Pa. 19140

Full-thickness explants of rabbit cornea were grown in organ culture and the migratory behavior of both epithelium and endothelium was observed under different experimental conditions. Following explantation, epithelial migration along the cut surface of the stroma began within six hours. By 24 hours, the epithelium reached the cut edge of Descemet's membrane and made contact with the endothelium at this point. Further epithelial advancement was then completely inhibited for up to 30 days following contact with the endothelium. Likewise, there was no migratory endothelial movement. If the endothelium was mechanically removed from the posterior corneal surface just prior to culture, the epithelium subsequently moved onto Descemet's membrane and completely encircled the explant after only 48 hours in vitro. Prior removal of epithelium permitted the endothelium to behave in a similar manner. However, endothelial migration was not completed until 96 hours in vitro because of a 48-hour delay before movement began. The results show that epithelium and endothelium mutually inhibit each other's forward movement and suggest an important role for this inhibitory interaction during corneal wound healing.

Key Words: organ culture • corneal epithelium • corneal endothelium • contact inhibition • corneal healing

Submitted on March 1, 1974




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Br. J. Ophthalmol., January 1, 2004; 88(1): 152 - 153.
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