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(Investigative Ophthalmology and Visual Science. 1974;13:593-601.)
© 1974 by The Association for Research in Vision and Ophthalmology, Inc.

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Ultrastructural Studies of Human Adenovirus-Produced Retinoblastoma-like Neoplasms in Sprague-Dawley Rats

NORITSUGU MUKAI 1, SHOP KOBAYASHI 1, and MASAMI OGURI 2

1 Wesley C. Bowers, Laboratory of Pharmacology and Experimental Pathology, Department of Retina Research, Retina Foundation Boston, Mass. 02114
2 Wesley C. Bowers Laboratory of Pharmacology and Experimental Pathology, Department of Retina Research, Retina Foundation Boston, Mass. 02114

Sixteen out of 85 Sprague-Dawley rats (18.8 per cent) developed solid medullary tumors in the left ocular cavity within 74 to 288 days after a single intravitreous inoculation of 0.015 to 0.03 ml. of fluid containing human adenovirus type 12, 103.5 to 104.5 TCID50 HeLa cells per 0.1 ml. The left eye was inoculated within 24 hours after birth. Tumor-bearing rats were perfused with Karnovsky's fixative and their enucleated eyes were subjected to epon-embedded thin sectioning. The remarkably uniform microscopic appearance of all cases resembled human retinoblastoma with incomplete rosettes. Ultrastructurally, the tumor cells forming incomplete rosettes and perivascular wreaths possessed oval or slightly indented nuclei without noticeable nuclear membrane abnormalities. A slender luminal pole of cytoplasm in each cell contained poorly differentiated organelles. One cilium per cell with a 9 + 0 pattern of doublets associated with a pair of centrioles was detectable in all cases. A strong tendency for many tumor cells to undergo bizarre ganglioneuronic maturation was also characteristic.

Note:
Part of this work was presented at the Joint Meeting of the American Association of Neuropathologists, Pediatric Pathology Club, and American Association of Pathologists and Bacteriologists held at the San Francisco Hilton Hotel on March 9, 1974.

Key Words: human adenovirus type 12 • retinoblastoma-like neoplasms • incomplete rosettes • unipolar tumor cells • ganglioneuronic differentiation

Submitted on June 9, 1974




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N Mukai, S. Kalter, L. Cummins, V. Matthews, T Nishida, and T Nakajima
Retinal tumor induced in the baboon by human adenovirus 12
Science, November 28, 1980; 210(4473): 1023 - 1025.
[Abstract] [PDF]


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Copyright © 1974 by the Association for Research in Vision and Ophthalmology