IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chylack, L. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chylack, L. T., Jr

Investigative Ophthalmology & Visual Science, Vol 14, 854-862, Copyright © 1975 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

The characterization of the hexokinases in several tissues of the calf eye

LT Chylack Jr

Several tissues from the calf eye were studied so as to determine the characteristics of the hexokinases in each tissue. The corneal stroma, sclera, and vitreous body are devoid of measurable hexokinase activity. The tissue specific activities decrease in the following order: retina, ciliary body, corneal endothelium, lens capsule-epithelium, corneal epithelium, and lens cortex-nucleus. Each tissue possesses Types I and II hexokinase in varying proportions in the soluble and insoluble fractions; latent hexokinase is Type I in all tissues except the retina in which both Types I and II are found in the latent fraction. No hexokinase Type IIa, III, or IV is found in any ocular tissue. Michaelis constants for glucose and MgATP= are similar to those found in extraocular tissues. Except for retinal hexokinase, ocular hexokinases are quite similar to extraocular hexokinases. The activity of retinal hexokinase varies greatly and abruptly with time and with change in buffer composition; in the absence of Na2EDTA, glucose, and - SH compounds, there is a rapid deactivation of hexokinase and a shift of the soluble to the insoluble form. The relevance of these findings to glycolysis in each tissue is discussed.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1975 by the Association for Research in Vision and Ophthalmology