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Investigative Ophthalmology & Visual Science, Vol 14, 947-950, Copyright © 1975 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Analysis of ascorbate concentration in the aqueous humor by high- pressure liquid chromatography

KW Lam and PF Lee

A high-pressure liquid chromatography instrument has been used to establish a method for the analyses of ascorbate concentration in aqueous humor. The method detects only the reduced form of ascorbate. The values obtained by this method are very similar to those of the colorimetric method. The ascorbate value in a group of random samples obtained from human glaucoma eyes varied between 3.5 to 31 mg. per cent, independent to blood ascorbate concentration.




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Copyright © 1975 by the Association for Research in Vision and Ophthalmology