| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Investigative Ophthalmology & Visual Science, Vol 21, 135-142, Copyright © 1981 by Association for Research in Vision and Ophthalmology
ARTICLES AND REPORTS |
MS Shapiro, J Friend and RA Thoft
After debridement of the entire corneal epithelium, epithelial cells of conjunctival origin cover the exposed corneal surface. Four to five weeks later, these cells undergo a morphologic transformation to normal- appearing corneal epithelium. To study this transformation the entire corneal epithelium was removed from rabbits with the use of n-heptanol, after which the histologic appearance of and the number of goblet cells in the regenerated epithelium were noted. Five stages of transformation were seen. Immediately after healing, the epithelium consisted of one to two squamous cell layers with no goblet cells apparent at the light microscope level (stage 1). In the following weeks goblet cells appeared at the limbal edge of the cornea (stage 2), reached a uniform distribution across the cornea (stage 3), and subsequently receded toward the limbus (stage 4), leaving an epithelium with normal corneal morphologic appearance (stage 5). To see if there was an ongoing centripetal cell migration from the conjunctiva across the cornea after initial healing, the central corneal epithelium was isolated from the periphery by a ring of glue. Such isolation resulted in a thinning of the central epithelium and a thickening of the peripheral epithelium. These studies suggest that (1) the transformation into corneal epithelium lags behind defect closure by 4 to 5 weeks, (2) goblet cells do not initially migrate as recognizable cells, and (3) there is a continuous centripetal cell motion even after the initial defect closure is accomplished.
This article has been cited by other articles:
|
|
 |
|
  L. P. K. Ang, T. Nakamura, T. Inatomi, C. Sotozono, N. Koizumi, N. Yokoi, and S. Kinoshita Autologous serum-derived cultivated oral epithelial transplants for severe ocular surface disease. Arch Ophthalmol, November 1, 2006; 124(11): 1543 - 1551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kawasaki, H. Tanioka, K. Yamasaki, N. Yokoi, A. Komuro, and S. Kinoshita Clusters of corneal epithelial cells reside ectopically in human conjunctival epithelium. Invest. Ophthalmol. Vis. Sci., April 1, 2006; 47(4): 1359 - 1367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nakamura, L. P. K. Ang, H. Rigby, E. Sekiyama, T. Inatomi, C. Sotozono, N. J. Fullwood, and S. Kinoshita The use of autologous serum in the development of corneal and oral epithelial equivalents in patients with stevens-johnson syndrome. Invest. Ophthalmol. Vis. Sci., March 1, 2006; 47(3): 909 - 916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hayashida, K. Nishida, M. Yamato, K. Watanabe, N. Maeda, H. Watanabe, A. Kikuchi, T. Okano, and Y. Tano Ocular Surface Reconstruction Using Autologous Rabbit Oral Mucosal Epithelial Sheets Fabricated Ex Vivo on a Temperature-Responsive Culture Surface Invest. Ophthalmol. Vis. Sci., May 1, 2005; 46(5): 1632 - 1639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Nakamura, T Inatomi, C Sotozono, T Amemiya, N Kanamura, and S Kinoshita Transplantation of cultivated autologous oral mucosal epithelial cells in patients with severe ocular surface disorders Br. J. Ophthalmol., October 1, 2004; 88(10): 1280 - 1284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Pal-Ghosh, A. Pajoohesh-Ganji, M. Brown, and M. A. Stepp A Mouse Model for the Study of Recurrent Corneal Epithelial Erosions: {alpha}9{beta}1 Integrin Implicated in Progression of the Disease Invest. Ophthalmol. Vis. Sci., June 1, 2004; 45(6): 1775 - 1788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nakamura, K.-I. Endo, L. J. Cooper, N. J. Fullwood, N. Tanifuji, M. Tsuzuki, N. Koizumi, T. Inatomi, Y. Sano, and S. Kinoshita The Successful Culture and Autologous Transplantation of Rabbit Oral Mucosal Epithelial Cells on Amniotic Membrane Invest. Ophthalmol. Vis. Sci., January 1, 2003; 44(1): 106 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Joussen, V. Poulaki, N. Mitsiades, S. U. Stechschulte, B. Kirchhof, D. A. Dartt, G.-H. Fong, J. Rudge, S. J. Wiegand, G. D. Yancopoulos, et al. VEGF-Dependent Conjunctivalization of the Corneal Surface Invest. Ophthalmol. Vis. Sci., January 1, 2003; 44(1): 117 - 123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Koizumi, L. J. Cooper, N. J. Fullwood, T. Nakamura, K. Inoki, M. Tsuzuki, and S. Kinoshita An Evaluation of Cultivated Corneal Limbal Epithelial Cells, Using Cell-Suspension Culture Invest. Ophthalmol. Vis. Sci., July 1, 2002; 43(7): 2114 - 2121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Koizumi, N. J. Fullwood, G. Bairaktaris, T. Inatomi, S. Kinoshita, and A. J. Quantock Cultivation of Corneal Epithelial Cells on Intact and Denuded Human Amniotic Membrane Invest. Ophthalmol. Vis. Sci., August 1, 2000; 41(9): 2506 - 2513. [Abstract] [Full Text]
|
|
|
|
|
|
W. Philipp, L. Speicher, and C. Humpel Expression of Vascular Endothelial Growth Factor and Its Receptors in Inflamed and Vascularized Human Corneas Invest. Ophthalmol. Vis. Sci., August 1, 2000; 41(9): 2514 - 2522. [Abstract] [Full Text]
|
|
|
|
|
|
G. Langer, W. Jagla, W. Behrens-Baumann, S. Walter, and W. Hoffmann Secretory Peptides TFF1 and TFF3 Synthesized in Human Conjunctival Goblet Cells Invest. Ophthalmol. Vis. Sci., September 1, 1999; 40(10): 2220 - 2224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. S Dua The conjunctiva in corneal epithelial wound healing Br. J. Ophthalmol., December 1, 1998; 82(12): 1407 - 1411. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Lehrer, T. Sun, and R. Lavker Strategies of epithelial repair: modulation of stem cell and transit amplifying cell proliferation J. Cell Sci., January 10, 1998; 111(19): 2867 - 2875. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
