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Investigative Ophthalmology & Visual Science, Vol 22, 478-481, Copyright © 1982 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

The enucleated arterially perfused cat eye. I. Effects on protein permeability

FJ Macri, SJ Cevario and JE Pederson

Protein permeability of the ciliary epithelium of enucleated arterially perfused cat eyes was studied. Tracer concentrations of 125I-albumin, in an arterial perfusate solution consisting of a balanced salt solution (BSS) without added colloid, was found in the anterior chamber fluid (AH) in a percentage similar to the AH/plasma ratio found for protein in the living cat eye. Stimulation of AH formation by acetylcholine plus eserine increased protein permeability markedly and by a similar amount in eyes perfused either with BSS or with 100% serum. These data indicate that arterial perfusion of the enucleated eye with BSS alone induces no gross abnormality of the blood-aqueous barrier.




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Copyright © 1982 by the Association for Research in Vision and Ophthalmology