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Investigative Ophthalmology & Visual Science, Vol 22, 808-811, Copyright © 1982 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

13C-nuclear magnetic resonance studies of sugar cataractogenesis in the single intact rabbit lens

RG Gonzalez, J Willis, J Aguayo, P Campbell, LT Chylack Jr and T Schleich

In this report we demonstrate that 13C-nuclear magnetic resonance spectroscopy may be used to monitor the sorbitol and glycolytic pathways in a single, intact, crystalline lens of the rabbit, thereby providing a new, noninvasive method for the study of sugar cataractogenesis. This approach is particularly useful for the measurement of intralenticular metabolic fluxes in single lenses under various conditions, at a time resolution previously unavailable in lens biochemistry.


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W. Williams and J. Odom
Study of aldose reductase inhibition in intact lenses by 13C nuclear magnetic resonance spectroscopy
Science, July 11, 1986; 233(4760): 223 - 225.
[Abstract] [PDF]


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Copyright © 1982 by the Association for Research in Vision and Ophthalmology