IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Greiner, J. V.
Right arrow Articles by Glonek, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Greiner, J. V.
Right arrow Articles by Glonek, T.

Investigative Ophthalmology & Visual Science, Vol 23, 14-22, Copyright © 1982 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Dynamic changes in the organophosphate profile upon treatment of the crystalline lens with dexamethasone

JV Greiner, SJ Kopp and T Glonek

Dynamic changes in organophosphate metabolites during incubations of the intact crystalline lens in a medium containing 2 X 10(-3) M dexamethasone were studied using phosphorus-31 nuclear magnetic resonance intact-tissue spectroscopy. ATP, ADP, inorganic orthophosphate, alpha-glycerophosphate, nicotinamide adenine dinucleotides, uridine diphosphorylglucose and diphosphorylgalactose, glycerol 3-phosphorylethanolamine and -phosphorylcholine, and an unidentified phosphorus-containing molecule at 6 ppm were quantitated. Time-dependent changes in lens metabolism involving these compounds were examined during a 24-hr incubation period with dexamethasone. The first event in the decline of the tissue after steroid incubation was the consumption of ATP, which was linear with time until the ATP was reduced to 10% of its initial value. The sugar phosphates, inorganic orthophosphate, and the nucleoside diphosphorylsugars increased linearly with time, whereas the dinucleotides decreased. The ADP concentration was unaffected by the steroid treatment, as was that of the other organophosphates in the intact tissue profile. The steroid- induced alterations in lens phosphate metabolite levels follow a distinctive pattern that differs substantially from that observed in response to experimental hypoglycistic or hyperglycistic conditions. These findings suggest that pharmacologic steroid doses may directly alter lens metabolism through a complex mechanism involving, at least in part, a steroid-mediated antagonism of lens glucose uptake and/or utilization.


This article has been cited by other articles:


Home page
 IOVSHome page
O. Risa, O. Saether, S. Lofgren, P. G. Soderberg, J. Krane, and A. Midelfart
Metabolic Changes in Rat Lens after In Vivo Exposure to Ultraviolet Irradiation: Measurements by High Resolution MAS 1H NMR Spectroscopy
Invest. Ophthalmol. Vis. Sci., June 1, 2004; 45(6): 1916 - 1921.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. I. Jobling, A. Stevens, and R. C. Augusteyn
Binding of Dexamethasone by {{alpha}}-Crystallin
Invest. Ophthalmol. Vis. Sci., July 1, 2001; 42(8): 1829 - 1832.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
W. Williams and J. Odom
Study of aldose reductase inhibition in intact lenses by 13C nuclear magnetic resonance spectroscopy
Science, July 11, 1986; 233(4760): 223 - 225.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1982 by the Association for Research in Vision and Ophthalmology