IOVS Molecular Pharmacology
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Investigative Ophthalmology & Visual Science, Vol 23, 41-49, Copyright © 1982 by Association for Research in Vision and Ophthalmology


ARTICLES AND REPORTS

Corneal epithelial cell cultures on stromal carriers

J Friend, S Kinoshita, RA Thoft and JA Eliason

Exposure of denuded rabbit corneal stromal carriers for 24 hr at 37 degrees C to suspensions of rabbit corneal epithelial cells grown in tissue culture resulted in the establishment of a cell layer on the carriers. The cell layers persisted for at least 1 week of incubation and were one to three cells thick. They consisted of healthy-appearing cells with normal intracellular organelles and intercellular desmosomal connections. After 2 to 7 days of incubation the cells were still capable of DNA replication and produced hemidesmosomes and basement membrane. This system is useful for in vitro studies of substrate requirements for hemidesmosome and basement-membrane formation by corneal epithelial cells.


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L. Taliana, M. D. M. Evans, S. D. Dimitrijevich, and J. G. Steele
The Influence of Stromal Contraction in a Wound Model System on Corneal Epithelial Stratification
Invest. Ophthalmol. Vis. Sci., January 1, 2001; 42(1): 81 - 89.
[Abstract] [Full Text]


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N. Koizumi, N. J. Fullwood, G. Bairaktaris, T. Inatomi, S. Kinoshita, and A. J. Quantock
Cultivation of Corneal Epithelial Cells on Intact and Denuded Human Amniotic Membrane
Invest. Ophthalmol. Vis. Sci., August 1, 2000; 41(9): 2506 - 2513.
[Abstract] [Full Text]




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