IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Worthen, D. M.
Right arrow Articles by Cleveland, P. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Worthen, D. M.
Right arrow Articles by Cleveland, P. H.

Investigative Ophthalmology & Visual Science, Vol 23, 265-269, Copyright © 1982 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Fibronectin production by cultured human trabecular meshwork cells

DM Worthen and PH Cleveland

Postmortem explants of human trabecular meshwork from 23 eyebank eyes had a growth rate of 32% (71/160) cultures, with 8% (12) showing abundant cellular spread. One hundred percent confluency was achieved with eyes from donors of 11 to 40 years of age. One of the cultures grew profusely and we were able to study it through eight passages in culture. This culture had the morphologic characteristics described by Polansky et al. for cultured human trabecular meshwork cells. A sensitive radioimmunoassay for fibronectin in tissue culture supernatant showed an increase in fibronectin secretion over a 96 hr period, with a characteristic shift-down response occurring between 24 and 48 hr postmedia change. Three of four cultures showed the presence of laminin, a basement membrane protein, establishing that the cells are not fibroblasts.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1982 by the Association for Research in Vision and Ophthalmology