Investigative Ophthalmology & Visual Science, Vol 23, 646-650, Copyright © 1982 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Cytochalasin B and D dose-outflow facility response relationships in the cynomolgus monkey

PL Kaufman and KA Erickson

Total outflow facility was determined by two-level constant-pressure perfusion in cynomolgus monkeys for 30 to 40 min before and after intracameral infusion of cytochalasins B and D (CB, CD). CB and CD dose- facility response relationships were defined. CB doses of 0.1, 0.2, and 0.5 micrograms were subthreshold; 1, 2, 5 and 10 micrograms were effective, with 5 micrograms probably supramaximal. CD at 0.02 microgram was probably subthreshold; 0.1, 0.5, 1, 2.5, and 5 micrograms doses were effective, with 0.1 microgram being near or above maximum. Although the maximum effects of both agents were quantitatively similar, approximately doubling-starting facility, CD Was about 25 times more potent. Therefore, CB- and CD-induced facility increases are presumably due largely to interference with formation, structure, and function of actin filaments in the endothelial cells of the trabecular meshwork and inner wall of Schlemm's canal.

This article has been cited by other articles:

				H. Sakai, B.-C. Park, X. Shen, and B. Y. J. T. Yue Transduction of TAT Fusion Proteins into the Human and Bovine Trabecular Meshwork. Invest. Ophthalmol. Vis. Sci., October 1, 2006; 47(10): 4427 - 4434. [Abstract] [Full Text] [PDF]

				J. Song, P.-F. Deng, S. S. Stinnett, D. L. Epstein, and P. V. Rao Effects of Cholesterol-Lowering Statins on the Aqueous Humor Outflow Pathway Invest. Ophthalmol. Vis. Sci., July 1, 2005; 46(7): 2424 - 2432. [Abstract] [Full Text] [PDF]

				A. J. Santas, C. Bahler, J. A. Peterson, M. S. Filla, P. L. Kaufman, E. R. Tamm, D. H. Johnson, and D. M. P. Peters Effect of Heparin II Domain of Fibronectin on Aqueous Outflow in Cultured Anterior Segments of Human Eyes Invest. Ophthalmol. Vis. Sci., November 1, 2003; 44(11): 4796 - 4804. [Abstract] [Full Text] [PDF]

				Y. Nakamura, T. Sagara, K. Seki, S. Hirano, and T. Nishida Permissive Effect of Fibronectin on Collagen Gel Contraction Mediated by Bovine Trabecular Meshwork Cells Invest. Ophthalmol. Vis. Sci., October 1, 2003; 44(10): 4331 - 4336. [Abstract] [Full Text] [PDF]

				B. Tian, J. A. Kiland, and P. L. Kaufman Effects of the Marine Macrolides Swinholide A and Jasplakinolide on Outflow Facility in Monkeys Invest. Ophthalmol. Vis. Sci., December 1, 2001; 42(13): 3187 - 3192. [Abstract] [Full Text] [PDF]

				P. V. Rao, P.-F. Deng, J. Kumar, and D. L. Epstein Modulation of Aqueous Humor Outflow Facility by the Rho Kinase-Specific Inhibitor Y-27632 Invest. Ophthalmol. Vis. Sci., April 1, 2001; 42(5): 1029 - 1037. [Abstract] [Full Text]

				J. Ueda, K. K. Wentz–Hunter, E. L. Cheng, T. Fukuchi, H. Abe, and B. Y.J.T. Yue Ultrastructural Localization of Myocilin in Human Trabecular Meshwork Cells and Tissues J. Histochem. Cytochem., October 1, 2000; 48(10): 1321 - 1330. [Abstract] [Full Text]

				K. A. Erickson and A. Schroeder Direct Effects of Muscarinic Agents on the Outflow Pathways in Human Eyes Invest. Ophthalmol. Vis. Sci., June 1, 2000; 41(7): 1743 - 1748. [Abstract] [Full Text]

				B. Tian, B. Geiger, D. L. Epstein, and P. L. Kaufman Cytoskeletal Involvement in the Regulation of Aqueous Humor Outflow Invest. Ophthalmol. Vis. Sci., March 1, 2000; 41(3): 619 - 623. [Full Text]

				L. A. Al-Aswad, H. Gong, D. Lee, M. E. O’Donnell, J. D. Brandt, W. J. Ryan, A. Schroeder, and K. A. Erickson Effects of Na-K-2Cl Cotransport Regulators on Outflow Facility in Calf and Human Eyes In Vitro Invest. Ophthalmol. Vis. Sci., July 1, 1999; 40(8): 1695 - 1701. [Abstract] [Full Text] [PDF]

				B. Tian, P. L. Kaufman, T. Volberg, B'A. T. Gabelt, and B. Geiger H-7 Disrupts the Actin Cytoskeleton and Increases Outflow Facility Arch Ophthalmol, May 1, 1998; 116(5): 633 - 643. [Abstract] [Full Text] [PDF]