IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kennedy, A.
Right arrow Articles by Varma, S. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kennedy, A.
Right arrow Articles by Varma, S. D.

Investigative Ophthalmology & Visual Science, Vol 24, 1250-1258, Copyright © 1983 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Aldose reductase activity in retinal and cerebral microvessels and cultured vascular cells

A Kennedy, RN Frank and SD Varma

Isolated microvessels (primarily capillaries) from bovine retina and cerebral cortex, as well as cultured bovine retinal capillary pericytes and porcine and canine retinal capillary endothelial cells contain apparent aldose reductase activity. This conclusion is based on the ability of these cultured cells and vessel fragments to reduce DL- glyceraldehyde in preference to D-glucuronate at low (0.1 mM) substrate concentrations, in the presence of NADPH, and in the accumulation of high levels of sorbitol or galactitol when retinal pericytes and endothelial cells are cultured in media enriched in glucose or galactose. The quantitative similarities of these activities in bovine retinal and cerebral microvessels, as well as the quantitatively similar ability of these two sets of microvessels to oxidize 14C- labeled glucose with the label either in the C-1 or the C-6 position, suggests that aldose reductase may not be a major causal factor in diabetic retinopathy. This conclusion is suggested because, while these metabolic activities are similar in bovine retinal and cerebral microvessels, only the retinal microvasculature suffers major anatomic and functional damage in diabetes. This conclusion must be viewed with caution, however, because other metabolic pathways that we have not investigated may be altered by an excess of sugar alcohols, and be present in differing activities in retinal and cerebral microvessels; species differences may exist; and similar experiments have not been conducted using human microvessels.


This article has been cited by other articles:


Home page
 VASC ENDOVASCULAR SURGHome page
K. G. Maier
Nicotinamide Adenine Dinucleotide Phosphate Oxidase and Diabetes: Vascular Implications
Vascular and Endovascular Surgery, August 1, 2008; 42(4): 305 - 313.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. A. Nadal, G. M. Scicli, L. A. Carbini, and A. G. Scicli
Angiotensin II stimulates migration of retinal microvascular pericytes: involvement of TGF-beta and PDGF-BB
Am J Physiol Heart Circ Physiol, February 1, 2002; 282(2): H739 - H748.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
C. Yabe-Nishimura
Aldose Reductase in Glucose Toxicity: A Potential Target for the Prevention of Diabetic Complications
Pharmacol. Rev., March 1, 1998; 50(1): 21 - 34.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1983 by the Association for Research in Vision and Ophthalmology