IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Flannery, J. G.
Right arrow Articles by Fisher, S. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Flannery, J. G.
Right arrow Articles by Fisher, S. K.

Investigative Ophthalmology & Visual Science, Vol 25, 229-232, Copyright © 1984 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Circadian disc shedding in Xenopus retina in vitro

JG Flannery and SK Fisher

To further examine the endogenous rhythm of disc shedding and phagocytosis observed in several species, adult Xenopus were entrained to a 12 hr light/12 hr dark cycle and then placed in constant darkness. At various times during a 3-day period of constant darkness, eyes were explanted and placed into culture medium, then processed for light and electron microscopy. A clear rhythmicity of disc shedding was observed, with pronounced peaks at the times light onset occurred in the original entrainment cycle. Modification of the HCO3- ion concentration in the medium was found to raise the amplitude of the peak of endogenous disc shedding. Explants maintained in culture medium containing deuterium oxide (a compound known to perturb circadian oscillators) were found to shed with a longer interval between peaks. The addition of the protein synthesis inhibitor, anisomycin, to this preparation suppressed the shedding rhythm. The action of anisomycin was investigated by autoradiographic examination of the pattern of 3H-leucine uptake and protein synthesis by the explant. The findings suggest the presence of a circadian oscillator for rhythmic disc shedding within the amphibian eye.


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
O. Strauss
The Retinal Pigment Epithelium in Visual Function
Physiol Rev, July 1, 2005; 85(3): 845 - 881.
[Abstract] [Full Text] [PDF]

Home page
 Arch Otolaryngol Head Neck SurgHome page
T. Kobayashi, T. Sato, M. Toshima, M. Ishidoya, M. Suetake, and T. Takasaka
Treatment of Labyrinthine Fistula With Interruption of the Semicircular Canals
Arch Otolaryngol Head Neck Surg, April 1, 1995; 121(4): 469 - 475.
[Abstract] [PDF]

Home page
 J Biol RhythmsHome page
C. E. Reme, A. Wirz-Justice, and M. Terman
The Visual Input Stage of the Mammalian Circadian Pacemaking System: I. Is There a Clock in the Mammalian Eye?
J Biol Rhythms, March 1, 1991; 6(1): 5 - 29.
[Abstract] [PDF]

Home page
 J Biol RhythmsHome page
H. Underwood, R. K. Barrett, and T. Siopes
The Quail's Eye: A Biological Clock
J Biol Rhythms, September 1, 1990; 5(3): 257 - 265.
[Abstract] [PDF]

Home page
 J Biol RhythmsHome page
H. Underwood, T. Siopes, and R. K. Barrett
Does a Biological Clock Reside in the Eye of Quail?
J Biol Rhythms, December 1, 1988; 3(4): 323 - 331.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1984 by the Association for Research in Vision and Ophthalmology