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Investigative Ophthalmology & Visual Science, Vol 25, 977-980, Copyright © 1984 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Purification of retinal S-antigen to homogeneity by the criterion of gel electrophoresis silver staining

JS Zigler Jr, M Mochizuki, T Kuwabara and I Gery

This report describes a procedure by which high performance liquid chromatographic (HPLC) techniques are used to obtain homogeneous S- antigen. Conventionally prepared S-antigen was purified further by fractionation on the anion exchange Mono-Q column followed by gel filtration on a TSK-3000 column. This procedure produced an S-antigen preparation, which appeared homogeneous by gel electrophoresis using the very sensitive silver staining method. The purified material retained its immunochemical and uveitogenic activity. The availability of homogeneous S-antigen will facilitate studies aimed at elucidating, at the molecular level, the mechanism by which S-antigen induces uveitis.




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Copyright © 1984 by the Association for Research in Vision and Ophthalmology