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Investigative Ophthalmology & Visual Science, Vol 25, 1007-1012, Copyright © 1984 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Monoclonal antibodies to Muller's cells of the retina

CC Chan, LA Rozenszajn, RB Nussenblatt, C Muellenberg-Coulombre, SM Hsu, AG Palestine, Z Lando and D BenEzra

The immunohistologic properties of two monoclonal antibodies produced by hybridomas generated from bovine retinal S-antigen (S-Ag) immunized mice were investigated. These monoclonal antibodies demonstrated a low antibody titer to the original S-Ag preparation by the ELISA method. Immunohistologic studies using avidin-biotin-peroxidase complex (ABC) showed strong specific binding to the retinal Muller cells of all species tested (human, bovine, guinea pig and rat), a weaker binding to cell bodies and proximal component of the outer segments of the photoreceptor, but no apparent binding to the distal component of the outer segments of the photoreceptor cells.




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Copyright © 1984 by the Association for Research in Vision and Ophthalmology