IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McLaren, J. W.
Right arrow Articles by Brubaker, R. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McLaren, J. W.
Right arrow Articles by Brubaker, R. F.

Investigative Ophthalmology & Visual Science, Vol 26, 144-152, Copyright © 1985 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

A two-dimensional scanning ocular fluorophotometer

JW McLaren and RF Brubaker

For certain studies of the dynamics of fluorescein in the anterior segment of the eye it would be advantageous to measure fluorescent intensity from several anatomic regions of the cornea and anterior chamber simultaneously or in rapid succession. In the present paper we describe a device called a two-dimensional scanning ocular fluorophotometer that we have designed and built to measure fluorescein concentration in a horizontal section through the cornea and anterior chamber. The 488-nm wavelength beam of an argon laser is mechanically scanned through the target area (70 microW total power at the eye) and fluorescent light is measured with a photon-counting photomultiplier tube. A single anterior-posterior scan requires 100 msec and is divided into 33 sample time periods of 3 msec each. Thirty anterior-posterior scans are made within 3 sec. A two-dimensional array of fluorescein concentration is reconstructed from the data. Concentrations ranging from 3 X 10(-10) gm/ml to 3 X 10(-6) g/ml are measured in a single scan. Examples of scans through the anterior chamber and cornea after topical and systemic administration of fluorescein are presented. These scans illustrate how this instrument can be used to measure two types of regional differences in fluorescein concentration, the "pupillary aqueous bubble" following topical administration and the radial concentration gradient in the cornea following systemic administration.


This article has been cited by other articles:


Home page
 IOVSHome page
A. J. Sit, C. B. Nau, J. W. McLaren, D. H. Johnson, and D. Hodge
Circadian Variation of Aqueous Dynamics in Young Healthy Adults
Invest. Ophthalmol. Vis. Sci., April 1, 2008; 49(4): 1473 - 1479.
[Abstract] [Full Text] [PDF]

Home page
 Br. J. Ophthalmol.Home page
A Steinfeld, A Lux, S Maier, R Suverkrup, and M Diestelhorst
Bioavailability of fluorescein from a new drug delivery system in human eyes
Br. J. Ophthalmol., January 1, 2004; 88(1): 48 - 53.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. W. McLaren, D. C. Herman, R. F. Brubaker, C. B. Nau, L. L. Wayman, M. G. Ciarniello, M. T. Rosignoli, and P. Dionisio
Effect of Ibopamine on Aqueous Humor Production in Normotensive Humans
Invest. Ophthalmol. Vis. Sci., November 1, 2003; 44(11): 4853 - 4858.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1985 by the Association for Research in Vision and Ophthalmology