IOVS AJP: Heart and Circulatory Physiology
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Investigative Ophthalmology & Visual Science, Vol 26, 1274-1280, Copyright © 1985 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Preparation of a uveitogenic peptide by chymotryptic digestion of bovine S-antigen

Y Kamada, M Yamada, ND Das, D Samuelson, VR Leverenz and H Shichi

Limited proteolysis of bovine S-antigen with alpha-chymotrypsin resulted in the accumulation of three peptides of MW 24,000, 16,000, and 12,000 daltons, respectively. By ELISA (enzyme-linked immunosorbent assay), MW 24,000 peptide was found to react with anti-S antibodies, but the other two peptides did not react with the antibodies under the assay conditions. The reactive peptide was separated from the smaller peptides by gel filtration on Sephadex G-75 and Sephadex G-50. When the MW 24,000 peptide was injected into Lewis rats, severe to mild uveitis was produced in all injected animals. The results indicate that the pathogenic determinant is on the MW 24,000 peptide.




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Copyright © 1985 by the Association for Research in Vision and Ophthalmology