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Investigative Ophthalmology & Visual Science, Vol 26, 1306-1309, Copyright © 1985 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Antigenic modulation in retinoblastoma: a flow cytometric study

NT Felberg, JJ Augsburger, JA Shields, J Goldschmidt, G Pronesti and A Haimowitz

Flow cytometry (FCM) was used to investigate antigenic expression and modulation during the cell cycle of Y-79 and WERI-Rb1 tissue cultured retinoblastoma cell lines using a polyclonal anti-Y-79 antibody and fluorescein conjugated lectins. Several Y-79 resting cell populations were identified by FCM analysis of antibody binding, while only a single population with uniform antigen expression was found to exist in the synthetic and mitotic phases. WERI-Rb1 cells bound antibody approximately equally in each phase of the cell cycle. Multiple cell populations with different lectin binding affinities were seen in the resting phase with FITC-concanavalin A, FITC-ricinus communis-60 and FITC-ricinus communis-120 (FITC-RCA-120). During the S-phase of the cell cycle, a higher percentage of cells bound FITC-RCA-120 and FITC wheat germ agglutinin. The relationship between antigenic expression during the cell cycle and treatment considerations in retinoblastoma is discussed.




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Copyright © 1985 by the Association for Research in Vision and Ophthalmology