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Investigative Ophthalmology & Visual Science, Vol 27, 840-844, Copyright © 1986 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Immunocytochemical localization of fibronectin to the retinal pigment epithelium of the rat

RM Pino

Fibronectin is an anionic asialoglycoprotein that is found on a variety of cell types. This study was undertaken in an attempt to localize fibronectin to the rat retinal pigment epithelium with ultrastructural immunocytochemistry. Using Fab-HRP conjugates specific for fibronectin, reaction product was localized on the surface of the apical processes and within the cell to GERL. After treatment of tissue by the biotin- avidin method employing ferritin-avidin, ferritin particles marked the apical processes in a quasi-regular distribution. Tufts of particles were separated by a linear distance of 65-85 nm. Fibronectin was not localized to rod outer segments.




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Copyright © 1986 by the Association for Research in Vision and Ophthalmology