IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tsukada, T.
Right arrow Articles by Chihara, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tsukada, T.
Right arrow Articles by Chihara, E.

Investigative Ophthalmology & Visual Science, Vol 27, 1115-1122, Copyright © 1986 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Changes in components of fast axonally transported proteins in the optic nerves of diabetic rabbits

T Tsukada and E Chihara

Proteins synthesized in retinal ganglion cells were labeled with 35S- methionine or 3H-leucine to the vitreous cavity, and the components of fast axonally transported proteins were studied in 20 nerves of 10 normal rabbits and 34 nerves of 17 alloxan-induced diabetic rabbits. Proteins in the optic nerves were separated by SDS-polyacrylamide gel electrophoresis, and axonally transferred proteins were studied by fluorography. In diabetic animals, the axonally transported radioactivity was one-fifth that of the controls. In the fluorographic spectrum of 35S-methionine-labeled proteins, relative proportion of 120kDalton(D)-proteins were increased by 43%, and 29kD proteins were decreased by 65% in diabetic rabbits. Relative proportion of other proteins, such as 24k, 36k, 68k, 150k and 180kD, were the same in control and diabetic rabbits. The quantitative reduction and change in spectrum of fast axonally transported proteins are compatible with the vulnerability and dysfunctions of nerve fibers in diabetic patients.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1986 by the Association for Research in Vision and Ophthalmology