| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Investigative Ophthalmology & Visual Science, Vol 28, 1559-1568, Copyright © 1987 by Association for Research in Vision and Ophthalmology
ARTICLES AND REPORTS |
KP Steuhl, G Doring, A Henni, HJ Thiel and K Botzenhart
Two pathogenic mechanisms of Pseudomonas aeruginosa corneal infections are discussed, one involving bacterial exoenzymes, the other involving polymorphonuclear leukocyte (PMN)-derived lysosomal enzymes. The objective of the present study was (1) to show the relative importance of the two mechanisms and (2) to evaluate the effect of active immunization against P. aeruginosa exoenzymes on ocular damage. Rabbits were immunized against P. aeruginosa alkaline protease (AP) or elastase (Ela) and challenged with the respective enzymes. Corneal damage was studied by light photography (LP). In another group, rabbits were immunized against AP, Ela and exotoxin A (ExoA) and challenged with P. aeruginosa strains PA01 or PA103. Corneal damage was studied with LP, light microscopy, and electron microscopy. Immunized animals were totally protected against intracorneal inoculation of P. aeruginosa proteases. Twelve hr and 24 hr after challenge with whole bacteria, immunized rabbits revealed less corneal damage than non-immunized animals. However, after 48 hr corneal damage (ie severe corneal ulceration) was comparable in both groups. The study suggests that corneal damage involving lysosomal enzymes from stimulated PMN is more important after bacterial infection than direct damage by P. aeruginosa exoenzymes.
This article has been cited by other articles:
|
|
 |
|
  Y. Kondo, K. Fukuda, T. Adachi, and T. Nishida Inhibition by a Selective I{kappa}B Kinase-2 Inhibitor of Interleukin-1-Induced Collagen Degradation by Corneal Fibroblasts in Three-Dimensional Culture Invest. Ophthalmol. Vis. Sci., November 1, 2008; 49(11): 4850 - 4857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. H. Hume, N. Cole, L. L. Garthwaite, S. Khan, and M. D. P. Willcox A Protective Role for IL-6 in Staphylococcal Microbial Keratitis Invest. Ophthalmol. Vis. Sci., November 1, 2006; 47(11): 4926 - 4930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Thakur, R. P. Barrett, J. A. Hobden, and L. D. Hazlett Caspase-1 Inhibitor Reduces Severity of Pseudomonas aeruginosa Keratitis in Mice Invest. Ophthalmol. Vis. Sci., September 1, 2004; 45(9): 3177 - 3184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang, H. Li, J. Wang, Z. Dong, S. Mian, and F.-S. X. Yu Role of EGFR Transactivation in Preventing Apoptosis in Pseudomonas aeruginosa-Infected Human Corneal Epithelial Cells Invest. Ophthalmol. Vis. Sci., August 1, 2004; 45(8): 2569 - 2576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Li, K. Fukuda, Y. Lu, Y. Nakamura, T.-i. Chikama, N. Kumagai, and T. Nishida Enhancement by neutrophils of collagen degradation by corneal fibroblasts J. Leukoc. Biol., September 1, 2003; 74(3): 412 - 419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Pillar and J. A. Hobden Pseudomonas aeruginosa Exotoxin A and Keratitis in Mice Invest. Ophthalmol. Vis. Sci., May 1, 2002; 43(5): 1437 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Kernacki, R. P Barrett, S. McClellan, and L. D. Hazlett MIP-1{alpha} regulates CD4+ T cell chemotaxis and indirectly enhances PMN persistence in Pseudomonas aeruginosa corneal infection J. Leukoc. Biol., December 1, 2001; 70(6): 911 - 919. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Dong, M. Ghabrial, M. Katar, R. Fridman, and R. S. Berk Membrane-Type Matrix Metalloproteinases in Mice Intracorneally Infected with Pseudomonas aeruginosa Invest. Ophthalmol. Vis. Sci., December 1, 2000; 41(13): 4189 - 4194. [Abstract] [Full Text]
|
|
|
|
|
|
L. D. Hazlett, S. McClellan, B. Kwon, and R. Barrett Increased Severity of Pseudomonas aeruginosa Corneal Infection in Strains of Mice Designated as Th1 versus Th2 Responsive Invest. Ophthalmol. Vis. Sci., March 1, 2000; 41(3): 805 - 810. [Abstract] [Full Text]
|
|
|
|
|
|
K. A. Kernacki, R. Barrett, and L. D. Hazlett Evidence for TIMP-1 Protection Against P. aeruginosa-Induced Corneal Ulceration and Perforation Invest. Ophthalmol. Vis. Sci., December 1, 1999; 40(13): 3168 - 3176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Cole, S. Bao, M. Willcox, and A. J. Husband Expression of Interleukin-6 in the Cornea in Response to Infection with Different Strains of Pseudomonas aeruginosa Infect. Immun., May 1, 1999; 67(5): 2497 - 2502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Hobden, S. Masinick-McClellan, R. P. Barrett, K. S. Bark, and L. D. Hazlett Pseudomonas aeruginosa Keratitis in Knockout Mice Deficient in Intercellular Adhesion Molecule 1 Infect. Immun., February 1, 1999; 67(2): 972 - 975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Kernacki, D. J. Goebel, M. S. Poosch, and L. D. Hazlett Early Cytokine and Chemokine Gene Expression during Pseudomonas aeruginosa Corneal Infection in Mice Infect. Immun., January 1, 1998; 66(1): 376 - 379. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
