Investigative Ophthalmology & Visual Science, Vol 29, 1836-1846, Copyright © 1988 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Trabecular meshwork cells grown on filters. Conductivity and cytochalasin effects

TW Perkins, JA Alvarado, JR Polansky, L Stilwell, M Maglio and R Juster
Department of Ophthalmology, University of California, San Francisco 94143.

A system was developed to measure the hydraulic conductivity of cultured monolayers of human trabecular meshwork (HTM) cells. By optimizing the cell growth conditions and evaluating a number of filter supports, confluent HTM cells in single layers were obtained for measurement of hydraulic conductivity. The HTM monolayers had hydraulic conductivities of 0.3-2.0 microliters/min/mm Hg/cm2 measured at near- physiological flow rates. Evaluations of cytochalasin B (CB) effects on the hydraulic conductivity of our HTM monolayers revealed that CB (10(- 6) to 10(-5) M) caused a dramatic dose-related increase in conductivity within 10 to 30 min, which parallels CB effects on outflow facility in vivo. Morphologic observations show that the increase in hydraulic conductivity was accompanied by a retraction of the trabecular cells and widening of the intercellular spaces. Our findings suggest that growth of HTM cells on filter supports can provide a useful in vitro system to study the regulation of aqueous outflow.

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