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(Investigative Ophthalmology and Visual Science. 1964;3:384-387.)
© 1964 by The Association for Research in Vision and Ophthalmology, Inc.

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Study of Lens Metabolism in vivo

Technique and Results with Na-24, Rb-86, and P-32

GEORGE E. GARCIA 1, THOMAS M. AABERG 1, and CARL KUPFER 1

1 Howe Laboratory of Ophthalmology, Harvard University Medical School, Massachusetts Eye and Ear Infirmary, Boston, Mass.

A technique for studying lens metabolism by anterior chamber perfusion in vivo is presented. A particular advantage of this technique is that the lens epithelium delineated by the pupillary space is the only portion of the lens surface exposed to the perfusate, there by providing in vivo isolation of this area. The uptake of P-32-labeled inorganic phosphate, Na-24, and Rb-86 by the lens to as investigated. The uptake of Rb-86 teas by far the greatest, while the uptake of P-32 and Na-24 was quantitatively similar despite the differences in their concentration gradients. The distribution of the isotopes in the lens, as determined by autoradiography, is also presented.






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