IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ueda, Y.
Right arrow Articles by Steinberg, R. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ueda, Y.
Right arrow Articles by Steinberg, R. H.

Investigative Ophthalmology & Visual Science, Vol 34, 3408-3418, Copyright © 1993 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Voltage-operated calcium channels in fresh and cultured rat retinal pigment epithelial cells

Y Ueda and RH Steinberg
Department of Physiology, UCSF School of Medicine 94143-0730.

PURPOSE. There is little known about the membrane properties of retinal pigment epithelial (RPE) cells with respect to calcium. The authors attempted to characterize membrane calcium channels from solitary fresh and cultured RPE cells from normal and dystrophic rat retinas. METHODS. RPE cells were enzymatically dissociated from eyes of neonatal rats of several strains, including dystrophic RCS strains. Membrane currents were recorded using the whole-cell version of the patch-clamp technique from either fresh or cultured cells. RESULTS. The authors observed sustained high-voltage-activated calcium channels that were dihydropyridine sensitive and closely resembled neuronal L-type calcium channels. The RCS-rdy+p+ strain was mainly investigated, but high- voltage-activated calcium channels were also recorded from fresh RPE cells of other rats regardless of age or strain, including RCS p+, RCS rdy+, Long Evans, Sprague Dawley, and also cultured RPE cells taken from a neonatal Long Evans strain. Low-voltage-activated calcium channels were not observed in any of these cells. CONCLUSION. Voltage- operated calcium channels of the L-type are the main calcium channels present in rat RPE cells. Cultured cells retained the identical channels. The dystrophic RCS strains (studied until 17 days postnatal) also exhibited these channels.


This article has been cited by other articles:


Home page
 J. Exp. Biol.Home page
L. M. Peltonen and S. Manttari
Is there life in the horny layer? Dihydropyridine and ryanodine receptors in the skin of female and male chickens (Gallus domesticus)
J. Exp. Biol., May 1, 2008; 211(9): 1394 - 1401.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
O. Strauss
The Retinal Pigment Epithelium in Visual Function
Physiol Rev, July 1, 2005; 85(3): 845 - 881.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
R. ROSENTHAL, H. THIEME, and O. STRAUSS
Fibroblast growth factor receptor 2 (FGFR2) in brain neurons and retinal pigment epithelial cells act via stimulation of neuroendocrine L-type channels (Cav1.3)
FASEB J, April 1, 2001; 15(6): 970 - 977.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
S. Mergler, K. Steinhausen, M. Wiederholt, and O. Strauss
Altered regulation of L-type channels by protein kinase C and protein tyrosine kinases as a pathophysiologic effect in retinal degeneration
FASEB J, September 1, 1998; 12(12): 1125 - 1134.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
C King-Smith, P Chen, D Garcia, H Rey, and B Burnside
Calcium-independent regulation of pigment granule aggregation and dispersion in teleost retinal pigment epithelial cells
J. Cell Sci., January 1, 1996; 109(1): 33 - 43.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the Association for Research in Vision and Ophthalmology