IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kahn, C. R.
Right arrow Articles by Rhim, J. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kahn, C. R.
Right arrow Articles by Rhim, J. S.

Investigative Ophthalmology & Visual Science, Vol 34, 3429-3441, Copyright © 1993 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Human corneal epithelial primary cultures and cell lines with extended life span: in vitro model for ocular studies

CR Kahn, E Young, IH Lee and JS Rhim
Gillette Medical Evaluation Laboratories, Gaithersburg, MD 20879.

PURPOSE. To develop an in vitro model of human corneal epithelium that can be propagated in serum-free medium that is tissue specific, species specific, and continuously available. METHODS. Primary explant cultures from human cadaver donor corneas were generated and subsequently infected with Adeno 12-SV40 (Ad12-SV40) hybrid virus or transfected with plasmid RSV-T. RESULTS. Several lines of human corneal epithelial cells with extended life span were developed and characterized. Propagation of both primary cultures and lines with extended life span, upon collagen membranes at an air-liquid interface, promoted multilayering, more closely approximating the morphology observed in situ. CONCLUSIONS. In vitro models, using primary cultures of corneal epithelium and lines of corneal epithelial cells with extended life span, retain a variety of phenotypic characteristics and may be used as an adjunct to ocular toxicology studies and as a tool to investigate corneal epithelial cell biology.


This article has been cited by other articles:


Home page
 IOVSHome page
D. M. Robertson, L. Li, S. Fisher, V. P. Pearce, J. W. Shay, W. E. Wright, H. D. Cavanagh, and J. V. Jester
Characterization of Growth and Differentiation in a Telomerase-Immortalized Human Corneal Epithelial Cell Line
Invest. Ophthalmol. Vis. Sci., February 1, 2005; 46(2): 470 - 478.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
G. Sosne, A. Siddiqi, and M. Kurpakus-Wheater
Thymosin-{beta}4 Inhibits Corneal Epithelial Cell Apoptosis after Ethanol Exposure In Vitro
Invest. Ophthalmol. Vis. Sci., April 1, 2004; 45(4): 1095 - 1100.
[Abstract] [Full Text] [PDF]

Home page
 Br. J. Ophthalmol.Home page
S Reichl, J Bednarz, and C C Muller-Goymann
Human corneal equivalent as cell culture model for in vitro drug permeation studies
Br. J. Ophthalmol., April 1, 2004; 88(4): 560 - 565.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
I. K. Gipson, S. Spurr-Michaud, P. Argueso, A. Tisdale, T. F. Ng, and C. L. Russo
Mucin Gene Expression in Immortalized Human Corneal-Limbal and Conjunctival Epithelial Cell Lines
Invest. Ophthalmol. Vis. Sci., June 1, 2003; 44(6): 2496 - 2506.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. V. Jester, J. Huang, S. Fisher, J. Spiekerman, J. H. Chang, W. E. Wright, and J. W. Shay
Myofibroblast Differentiation of Normal Human Keratocytes and hTERT, Extended-Life Human Corneal Fibroblasts
Invest. Ophthalmol. Vis. Sci., May 1, 2003; 44(5): 1850 - 1858.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
C. C. Chen, J.-H. Chang, J. B. Lee, J. Javier, and D. T. Azar
Human Corneal Epithelial Cell Viability and Morphology after Dilute Alcohol Exposure
Invest. Ophthalmol. Vis. Sci., August 1, 2002; 43(8): 2593 - 2602.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. McClellan, K. Howard, J. Y. Niederkorn, and H. Alizadeh
Effect of Steroids on Acanthamoeba Cysts and Trophozoites
Invest. Ophthalmol. Vis. Sci., November 1, 2001; 42(12): 2885 - 2893.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
E. Toropainen, V.-P. Ranta, A. Talvitie, P. Suhonen, and A. Urtti
Culture Model of Human Corneal Epithelium for Prediction of Ocular Drug Absorption
Invest. Ophthalmol. Vis. Sci., November 1, 2001; 42(12): 2942 - 2948.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
G. Geerling, J. T. Daniels, J. K. G. Dart, I. A. Cree, and P. T. Khaw
Toxicity of Natural Tear Substitutes in a Fully Defined Culture Model of Human Corneal Epithelial Cells
Invest. Ophthalmol. Vis. Sci., April 1, 2001; 42(5): 948 - 956.
[Abstract] [Full Text]

Home page
 IOVSHome page
X.-j. Yi, Y. Wang, and F.-S. X. Yu
Corneal Epithelial Tight Junctions and Their Response to Lipopolysaccharide Challenge
Invest. Ophthalmol. Vis. Sci., December 1, 2000; 41(13): 4093 - 4100.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
F Castro-Munozledo
Development of a spontaneous permanent cell line of rabbit corneal epithelial cells that undergoes sequential stages of differentiation in cell culture
J. Cell Sci., January 8, 1994; 107(8): 2343 - 2351.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the Association for Research in Vision and Ophthalmology