| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Investigative Ophthalmology & Visual Science, Vol 34, 413-419, Copyright © 1993 by Association for Research in Vision and Ophthalmology
ARTICLES AND REPORTS |
H Tanihara, M Yoshida, M Matsumoto and N Yoshimura
Department of Ophthalmology, Faculty of Medicine, Kyoto University, Japan.
PURPOSE. This study examined whether retinal pigment epithelial (RPE) cells have the capacity to express transforming growth factor-beta (TGF- beta). Also examined were TGF-beta isoform genes expressed in the RPE cells. METHODS. Complementary DNA (cDNA) was generated from polyA+ RNA extracted from human RPE cells in culture, and polymerase chain reaction (PCR) using degenerate and specific primers of the known TGF- beta isoforms was carried out by using the cDNAs as templates. Sequencing and Southern blot analysis of the PCR products, and Northern blot analysis were performed to identify which isoforms are expressed in RPE cells. RESULTS. PCR using degenerate primers showed that most of the amplified sequences were derived from TGF-beta 2, although TGF-beta 1 expression was shown by using specific primers. Northern blot analyses confirmed not only the expression of the TGF-beta gene, but also suggested that alternative splicing of TGF-beta mRNA in the RPE cells occurred. CONCLUSION. Cultured human RPE cells expressed TGF-beta 1 and beta 2 genes, whereas gene expression of TGF-beta 3 was not confirmed. Our data suggest that RPE cell are intraocular origin of TGF- beta production.
This article has been cited by other articles:
|
|
 |
|
  H. Lee, S. J. O'Meara, C. O'Brien, and R. Kane The Role of Gremlin, a BMP Antagonist, and Epithelial-to-Mesenchymal Transition in Proliferative Vitreoretinopathy Invest. Ophthalmol. Vis. Sci., September 1, 2007; 48(9): 4291 - 4299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Futagami, S. Sugita, J. Vega, K. Ishida, H. Takase, K. Maruyama, H. Aburatani, and M. Mochizuki Role of Thrombospondin-1 in T Cell Response to Ocular Pigment Epithelial Cells J. Immunol., June 1, 2007; 178(11): 6994 - 7005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sugita, H. Keino, Y. Futagami, H. Takase, M. Mochizuki, J. Stein-Streilein, and J. W. Streilein B7+ Iris Pigment Epithelial Cells Convert T Cells into CTLA-4+, B7-Expressing CD8+ Regulatory T Cells Invest. Ophthalmol. Vis. Sci., December 1, 2006; 47(12): 5376 - 5384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Strauss The Retinal Pigment Epithelium in Visual Function Physiol Rev, July 1, 2005; 85(3): 845 - 881. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Toyran, A Y Lin, and D P Edward Expression of growth differentiation factor-5 and bone morphogenic protein-7 in intraocular osseous metaplasia Br. J. Ophthalmol., July 1, 2005; 89(7): 885 - 890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Zamiri, S. Masli, N. Kitaichi, A. W. Taylor, and J. W. Streilein Thrombospondin Plays a Vital Role in the Immune Privilege of the Eye Invest. Ophthalmol. Vis. Sci., March 1, 2005; 46(3): 908 - 919. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. El Ghrably, D. G. Powe, G. Orr, D. Fischer, R. McIntosh, H. S. Dua, and P. J. Tighe Apoptosis in Proliferative Vitreoretinopathy Invest. Ophthalmol. Vis. Sci., May 1, 2004; 45(5): 1473 - 1479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sugita and J. W. Streilein Iris Pigment Epithelium Expressing CD86 (B7-2) Directly Suppresses T Cell Activation In Vitro via Binding to Cytotoxic T Lymphocyte-associated Antigen 4 J. Exp. Med., July 7, 2003; 198(1): 161 - 171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Callegan, M. Engelbert, D. W. Parke II, B. D. Jett, and M. S. Gilmore Bacterial Endophthalmitis: Epidemiology, Therapeutics, and Bacterium-Host Interactions Clin. Microbiol. Rev., January 1, 2002; 15(1): 111 - 124. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. ROSENTHAL, H. THIEME, and O. STRAUSS Fibroblast growth factor receptor 2 (FGFR2) in brain neurons and retinal pigment epithelial cells act via stimulation of neuroendocrine L-type channels (Cav1.3) FASEB J, April 1, 2001; 15(6): 970 - 977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wenkel and J. W. Streilein Evidence that Retinal Pigment Epithelium Functions as an Immune-Privileged Tissue Invest. Ophthalmol. Vis. Sci., October 1, 2000; 41(11): 3467 - 3473. [Abstract] [Full Text]
|
|
|
|
|
|
T. Udono, K. Takahashi, M. Nakayama, O. Murakami, Y. K. Durlu, M. Tamai, and S. Shibahara Adrenomedullin in Cultured Human Retinal Pigment Epithelial Cells Invest. Ophthalmol. Vis. Sci., June 1, 2000; 41(7): 1962 - 1970. [Abstract] [Full Text]
|
|
|
|
|
|
L. Carrington, D. McLeod, and M. Boulton IL-10 and Antibodies to TGF-{beta}2 and PDGF Inhibit RPE-Mediated Retinal Contraction Invest. Ophthalmol. Vis. Sci., April 1, 2000; 41(5): 1210 - 1216. [Abstract] [Full Text]
|
|
|
|
|
|
M. Yoshida, M. Takeuchi, and W. Streilein Participation of Pigment Epithelium of Iris and Ciliary Body in Ocular Immune Privilege. 1. Inhibition of T-Cell Activation In Vitro by Direct Cell-to-Cell Contact Invest. Ophthalmol. Vis. Sci., March 1, 2000; 41(3): 811 - 821. [Abstract] [Full Text]
|
|
|
|
|
|
U. Welge-Lu{beta}en, C. Wilsch, T. Neuhardt, J. W. Streilein, and E. Lutjen-Drecoll Loss of Anterior Chamber-Associated Immune Deviation (ACAID) in Aged Retinal Degeneration (rd) Mice Invest. Ophthalmol. Vis. Sci., December 1, 1999; 40(13): 3209 - 3214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Inatani, H. Tanihara, A. Oohira, M. Honjo, and Y. Honda Identification of a Nervous Tissue-Specific Chondroitin Sulfate Proteoglycan, Neurocan, in Developing Rat Retina Invest. Ophthalmol. Vis. Sci., September 1, 1999; 40(10): 2350 - 2359. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. G. T. Blaauwgeers, G. M. Holtkamp, H. Rutten, A. N. Witmer, P. Koolwijk, T. A. Partanen, K. Alitalo, M. E. Kroon, A. Kijlstra, V. W. M. van Hinsbergh, et al. Polarized Vascular Endothelial Growth Factor Secretion by Human Retinal Pigment Epithelium and Localization of Vascular Endothelial Growth Factor Receptors on the Inner Choriocapillaris : Evidence for a Trophic Paracrine Relation Am. J. Pathol., August 1, 1999; 155(2): 421 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hirase, T. Ikeda, C. Sotozono, K. Nishida, H. Sawa, and S. Kinoshita Transforming Growth Factor {beta}2 in the Vitreous in Proliferative Diabetic Retinopathy Arch Ophthalmol, June 1, 1998; 116(6): 738 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G CHARTERIS Growth factors in proliferative vitreoretinopathy Br. J. Ophthalmol., February 1, 1998; 82(2): 106 - 106. [Full Text]
|
|
|
|
|
|
M. Kliffen, H. S Sharma, C. M Mooy, S. Kerkvliet, and P. T V M de Jong Increased expression of angiogenic growth factors in age-related maculopathy Br. J. Ophthalmol., February 1, 1997; 81(2): 154 - 162. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
