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Investigative Ophthalmology & Visual Science, Vol 34, 477-487, Copyright © 1993 by Association for Research in Vision and Ophthalmology
ARTICLES AND REPORTS |
GA Lutty, C Merges, AB Threlkeld, S Crone and DS McLeod
Johns Hopkins School of Medicine, Baltimore, Maryland 21287-9115.
PURPOSE. The purpose of this work was to investigate further the immunohistochemical localization of transforming growth factor (TGF)- beta 1 to photoreceptors, which we had previously observed, to elucidate the cell types of origin for TGF-beta 1, and to determine sites of localization for TGF-beta 2 and beta 3 in the posterior segment of the human eye. METHOD. Streptavidin-peroxidase immunohistochemistry was used to localize TGF-beta antibodies in sections from cryopreserved human posterior segments from six adult donors. Two recently described polyclonal antibodies to TGF-beta 1 (anti-CC[1-30] and anti-LC[1-30]) and antibodies against TGF-beta 2 and beta 3 were employed in this study. Other studies suggest that anti- LC(1-30) antibody recognizes pre-pro-TGF-beta 1 and therefore sites of production, while anti-CC(1-30) recognizes secreted forms of TGF-beta 1. RESULTS. Regional labeling of choriocapillaris was observed in all eyes using anti-LC(1-30) (anti-pre-pro-beta 1). Anti-CC(1-30) (anti- secr-beta 1) was localized predominantly in photoreceptor inner and outer segments in all eyes. Outer segment localization appeared identical to anti-opsin localization, indicating that this form of TGF- beta 1 is most likely intracellular. TGF-beta 2 was observed predominantly in the connective tissue of long ciliary arteries in choroid and in photoreceptor outer segments in all eyes. TGF-beta 3 was observed in isolated individual cells in choroid and retina. Vitreous hyalocytes were the only cell type examined that were immunoreactive for all four forms of TGF-beta studied. CONCLUSION. The heterogeneity in localization of TGF-beta s in the human posterior eye suggests that TGF-beta s are produced, used, and degraded locally. TGF-beta 1 produced by the choriocapillaris may be stored or used by photoreceptors.
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