Investigative Ophthalmology & Visual Science, Vol 34, 2203-2209, Copyright © 1993 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Investigation of the role of the ras protooncogene point mutation in human uveal melanomas

CN Soparker, JM O'Brien and DM Albert
Department of Pathology, University of Massachusetts Medical Center, Worcester.

PURPOSE. Genetic alterations have been observed in a wide variety of neoplastic processes, including Burkitt's lymphoma, chronic myelogenous leukemia, promyelocytic leukemia, and solid tumors of the colon, skin, and breast. The polymerase chain reaction (PCR), dot blotting, and direct double-stranded DNA sequencing were used to assess ras gene activation in human uveal melanomas for three candidate genes: c-Ha- ras1, c-Ki-ras2, and N-ras at codons 12, 13, and 61. METHODS. Samples of 49 human uveal melanomas were obtained. Amplifiable high molecular weight DNA was obtained from 39 of these. PCR amplification of regions centering on three candidate ras genes was performed. PCR-amplified DNA was evaluated by dot blot and double-stranded DNA sequencing utilizing standard methods. RESULTS. No point mutations were identified in screening the c-Ha-ras gene nor were any genetic alterations found in the c-Ki-ras2 gene at codons 12 and 13. Only wild-type sequences were found at codon 61. No ras mutations were detected in any uveal melanomas studied. CONCLUSIONS. This study provides no evidence to support an association between ras protooncogene mutations and human uveal melanomas at codons 12, 13, or 61.

This article has been cited by other articles:

				M. D. Onken, L. A. Worley, M. D. Long, S. Duan, M. L. Council, A. M. Bowcock, and J. W. Harbour Oncogenic Mutations in GNAQ Occur Early in Uveal Melanoma Invest. Ophthalmol. Vis. Sci., December 1, 2008; 49(12): 5230 - 5234. [Abstract] [Full Text] [PDF]

				N. Babchia, A. Calipel, F. Mouriaux, A.-M. Faussat, and F. Mascarelli 17-AAG and 17-DMAG-Induced Inhibition of Cell Proliferation through B-Raf Downregulation in WTB-Raf-Expressing Uveal Melanoma Cell Lines Invest. Ophthalmol. Vis. Sci., June 1, 2008; 49(6): 2348 - 2356. [Abstract] [Full Text] [PDF]

				A. Calipel, F. Mouriaux, A.-L. Glotin, F. Malecaze, A.-M. Faussat, and F. Mascarelli Extracellular Signal-regulated Kinase-dependent Proliferation Is Mediated through the Protein Kinase A/B-Raf Pathway in Human Uveal Melanoma Cells J. Biol. Chem., April 7, 2006; 281(14): 9238 - 9250. [Abstract] [Full Text] [PDF]

				G. Lefevre, A.-L. Glotin, A. Calipel, F. Mouriaux, T. Tran, Z. Kherrouche, C.-A. Maurage, C. Auclair, and F. Mascarelli Roles of Stem Cell Factor/c-Kit and Effects of Glivec(R)/STI571 in Human Uveal Melanoma Cell Tumorigenesis J. Biol. Chem., July 23, 2004; 279(30): 31769 - 31779. [Abstract] [Full Text] [PDF]

				A. Calipel, G. Lefevre, C. Pouponnot, F. Mouriaux, A. Eychene, and F. Mascarelli Mutation of B-Raf in Human Choroidal Melanoma Cells Mediates Cell Proliferation and Transformation through the MEK/ERK Pathway J. Biol. Chem., October 24, 2003; 278(43): 42409 - 42418. [Abstract] [Full Text] [PDF]

				D. Rimoldi, S. Salvi, D. Lienard, F. J. Lejeune, D. Speiser, L. Zografos, and J.-C. Cerottini Lack of BRAF Mutations in Uveal Melanoma Cancer Res., September 15, 2003; 63(18): 5712 - 5715. [Abstract] [Full Text] [PDF]

				E. A. Hurst, J. W. Harbour, and L. A. Cornelius Ocular Melanoma: A Review and the Relationship to Cutaneous Melanoma Arch Dermatol, August 1, 2003; 139(8): 1067 - 1073. [Abstract] [Full Text] [PDF]

				Y. Cohen, N. Goldenberg-Cohen, P. Parrella, I. Chowers, S. L. Merbs, J. Pe'er, and D. Sidransky Lack of BRAF Mutation in Primary Uveal Melanoma Invest. Ophthalmol. Vis. Sci., July 1, 2003; 44(7): 2876 - 2878. [Abstract] [Full Text] [PDF]