IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yoshimura, N.
Right arrow Articles by Ishibashi, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshimura, N.
Right arrow Articles by Ishibashi, T.

Investigative Ophthalmology & Visual Science, Vol 36, 1686-1691, Copyright © 1995 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Photocoagulated human retinal pigment epithelial cells produce an inhibitor of vascular endothelial cell proliferation

N Yoshimura, M Matsumoto, H Shimizu, M Mandai, Y Hata and T Ishibashi
Department of Ophthalmology, Kyoto University, Faculty of Medicine, Japan.

PURPOSE. To determine if conditioned culture medium of photocoagulated human retinal pigment epithelial (RPE) cells contains inhibitors for the proliferation of bovine aortic endothelial cells (BAEC) and bovine retinal endothelial cells (BREC) and to characterize the nature of these inhibitory factors. METHODS. Retinal pigment epithelial cells grown to confluence were photocoagulated (0.1 second, 50 microns, 350 mW) in serum-free medium. After 48 hours, the conditioned medium (PC- CM) was removed, and the effects of non-acid-treated and transiently acid-treated samples were determined on [3H]-thymidine uptake by BAEC and BREC. PC-CM was also subjected to size exclusion high-performance liquid chromatography (HPLC). Fractions were analyzed for the effects on the growth of the bovine endothelial cells before and after transient acid treatment. RESULTS. The addition of non-acid-treated PC- CM (32% vol/vol) inhibited BREC [3H]-thymidine uptake to 18.5% of the control value. With HPLC, the inhibitory activity was recovered mainly in a fraction whose apparent molecular size was 25 kd. After transient acid treatment of the fractions, there also appeared a 100-kd inhibitor. Inhibitory effects were neutralized by pretreatment of the fractions with antiserum against transforming growth factor (TGF)-beta 2. CONCLUSIONS. Photocoagulated RPE cells secrete inhibitors of BAEC and BREC proliferation. Molecular size and immunologic properties of these inhibitors correspond to those of TGF-beta 2.


This article has been cited by other articles:


Home page
 Br. J. Ophthalmol.Home page
M Tretiach, M C Madigan, and M C Gillies
Conditioned medium from mixed retinal pigmented epithelium and Muller cell cultures reduces in vitro permeability of retinal vascular endothelial cells
Br. J. Ophthalmol., July 1, 2004; 88(7): 957 - 961.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
W. Schacke, K.-F. Beck, J. Pfeilschifter, F. Koch, and L.-O. Hattenbach
Modulation of Tissue Plasminogen Activator and Plasminogen Activator Inhibitor-1 by Transforming Growth Factor-{beta} in Human Retinal Glial Cells
Invest. Ophthalmol. Vis. Sci., August 1, 2002; 43(8): 2799 - 2805.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. X. Weng, L. Mo, H. L. Hellmich, A. S. L. Yu, T. Wood, and N. K. Wills
Expression and regulation of ClC-5 chloride channels: effects of antisense and oxidants
Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1511 - C1520.
[Abstract] [Full Text] [PDF]

Home page
 Br. J. Ophthalmol.Home page
J. Roider, R. Brinkmann, C. Wirbelauer, H. Laqua, and R. Birngruber
Subthreshold (retinal pigment epithelium) photocoagulation in macular diseases: a pilot study
Br. J. Ophthalmol., January 1, 2000; 84(1): 40 - 47.
[Abstract] [Full Text]

Home page
 Arch OphthalmolHome page
A. Oganesian, K. Gabrielian, J. T. Ernest, and S. C. Patel
A New Model of Retinal Pigment Epithelium Transplantation With Microspheres
Arch Ophthalmol, September 1, 1999; 117(9): 1192 - 1200.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
H. G. T. Blaauwgeers, G. M. Holtkamp, H. Rutten, A. N. Witmer, P. Koolwijk, T. A. Partanen, K. Alitalo, M. E. Kroon, A. Kijlstra, V. W. M. van Hinsbergh, et al.
Polarized Vascular Endothelial Growth Factor Secretion by Human Retinal Pigment Epithelium and Localization of Vascular Endothelial Growth Factor Receptors on the Inner Choriocapillaris : Evidence for a Trophic Paracrine Relation
Am. J. Pathol., August 1, 1999; 155(2): 421 - 428.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
J. Roider, R. Brinkmann, C. Wirbelauer, H. Laqua, and R. Birngruber
Retinal Sparing by Selective Retinal Pigment Epithelial Photocoagulation
Arch Ophthalmol, August 1, 1999; 117(8): 1028 - 1034.
[Abstract] [Full Text] [PDF]

Home page
 Br. J. Ophthalmol.Home page
W Y Shen, M J T Yu, C J Barry, I J Constable, and P E Rakoczy
Expression of cell adhesion molecules and vascular endothelial growth factor in experimental choroidal neovascularisation in the rat
Br. J. Ophthalmol., September 1, 1998; 82(9): 1063 - 1071.
[Abstract] [Full Text]

Home page
 Arch OphthalmolHome page
K. Hirase, T. Ikeda, C. Sotozono, K. Nishida, H. Sawa, and S. Kinoshita
Transforming Growth Factor {beta}2 in the Vitreous in Proliferative Diabetic Retinopathy
Arch Ophthalmol, June 1, 1998; 116(6): 738 - 741.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1995 by the Association for Research in Vision and Ophthalmology