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Investigative Ophthalmology & Visual Science, Vol 36, 1885-1892, Copyright © 1995 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Alpha-2 adrenergic receptors in the bovine retina. Presence of only the alpha-2D subtype

JR Berlie, LJ Iversen, HS Blaxall, ME Cooley, DM Chacko and DB Bylund
Department of Ophthalmology, University of Nebraska Medical Center, Omaha 68198-6260, USA.

PURPOSE. To identify and characterize the alpha-2 adrenergic receptor subtypes present in the bovine neurosensory retina. METHODS. Radioligand saturation and inhibition binding assays were performed with the antagonist radioligands [3H]RX821002 and [3H]rauwolscine. RESULTS. [3H]RX821002 bound to a single class of receptors with the characteristics of an alpha-2 adrenergic receptor with an affinity (KD) of 0.16 nM and a receptor density (Bmax) of 1500 fmol/mg protein. Correlation of the affinities (pKi values) for nine antagonists in the bovine neurosensory retina with the alpha-2D receptor of the bovine pineal gave a correlation coefficient of 0.99. The correlation coefficients for the alpha-2A (0.84), alpha-2B (0.36), and alpha-2C (0.39) subtypes were much lower. The presence of a minor population of alpha-2B or alpha-2C receptors was excluded. CONCLUSIONS. A high density of alpha-2D adrenergic receptors is present in the bovine neurosensory retina. Neither the alpha-2B nor the alpha-2C subtype is detectable.


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