IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yanagihara, N.
Right arrow Articles by Otani, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yanagihara, N.
Right arrow Articles by Otani, S.

Investigative Ophthalmology & Visual Science, Vol 37, 1975-1983, Copyright © 1996 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

The involvement of polyamines in the proliferation of cultured retinal pigment epithelial cells

N Yanagihara, M Moriwaki, K Shiraki, T Miki and S Otani
Department of Ophthalmology, Osaka City University Medical School, Japan.

PURPOSE: To investigate the involvement of polyamines (putrescine, spermidine, and spermine) in the proliferation of retinal pigment epithelium (RPE) using cultured bovine RPE cells. METHODS: The polyamine content and the activities of rate-limiting enzymes in polyamine biosynthesis (ornithine decarboxylase [ODC] and S- adenosylmethionine decarboxylase [SAMDC]) and in polyamine biodegradation (spermidine spermine N1-acetyltransferase [SAT]) were measured after proliferative stimulation by 10% fetal calf serum (FCS). DNA synthesis was determined by [3H]thymidine incorporation into the acid-insoluble fraction after the addition of an inhibitor of ODC (alpha-difluoromethylornithine [DFMO]) or SAMDC (methylglyoxal bis[guanylhydrazone] [MGBG]). The effects of exogenous polyamines on DNA synthesis after the additions of inhibitors also were determined. RESULTS: ODC and SAMDC activities were elevated after stimulation by FCS and reached their peaks 16 hours and 4 hours, respectively, after the addition of FCS. SAT activity was not increased. Polyamine content was increased significantly after stimulation by FCS. DFMO did not inhibit DNA synthesis induced by FCS, and only putrescine content was decreased significantly among polyamines. However, MGBG inhibited DNA synthesis in a dose-dependent manner, and the amounts of spermidine and spermine were decreased significantly. Exogenous polyamines, especially spermine, restored MGBG-induced inhibition of DNA synthesis. CONCLUSIONS: Polyamines are essential for the proliferation of cultured bovine RPE cells. These data suggest that, of the polyamines, spermine has the greatest effect on DNA synthesis although other polyamines can substitute for spermine at higher concentrations with similar results. As for polyamine metabolism in RPE proliferation, it is possible that SAMDC is the key enzyme rather than ODC.


This article has been cited by other articles:


Home page
 IOVSHome page
S. Kaneko, M. Ueda-Yamada, A. Ando, S. Matsumura, E. Okuda-Ashitaka, M. Matsumura, M. Uyama, and S. Ito
Cytotoxic Effect of Spermine on Retinal Pigment Epithelial Cells
Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 455 - 463.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
D. A. Johnson, C. Fields, A. Fallon, M. E. C. Fitzgerald, M. J. Viar, and L. R. Johnson
Polyamine-Dependent Migration of Retinal Pigment Epithelial Cells
Invest. Ophthalmol. Vis. Sci., April 1, 2002; 43(4): 1228 - 1233.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by the Association for Research in Vision and Ophthalmology