IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rada, J. A.
Right arrow Articles by Hassell, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rada, J. A.
Right arrow Articles by Hassell, J. R.

Investigative Ophthalmology & Visual Science, Vol 37, 2060-2067, Copyright © 1996 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Regionalized growth patterns of young chicken corneas

JA Rada, ME Fini and JR Hassell
Department of Anatomy and Cell Biology, University of North Dakota School of Medicine, Grand Forks 58202, USA.

PURPOSE: Extracellular matrix (ECM) accumulation, synthesis, and degradation were compared in central and peripheral corneal regions of 3-week-old chicks to identify regional differences within the cornea. METHODS: For collagen accumulation experiments, corneas were isolated at the scleral junction, the epithelium was removed, and the central corneal region was isolated from the peripheral region with a 3 mm trephine. After corneal stromas were digested with proteinase K, aliquots were used to estimate total collagen from hydroxyproline and cell number from DNA measurements. For biosynthesis experiments, corneas were placed in organ culture containing either in 3H-thymidine, 3H-proline, or 35SO4. After radiolabeling, the epithelium was removed, central and peripheral regions were isolated, and each corneal sample was analyzed for incorporated radioactivity. Gelatinolytic species present in each corneal region were compared qualitatively by gelatin zymography. RESULTS: Measurement of hydroxyproline content indicated that the central corneal stroma contained significantly more collagen per DNA than the peripheral corneal region (+40%, P = 0.013) and incorporated significantly higher levels of 3H-thymidine/ng DNA (+92%, P < 0.001), 3H-proline/ng DNA (+980%, P = 0.004), and 35SO4/ng DNA (+650%, P = 0.01) than the peripheral corneal region. Results of gelatin zymography indicated that the central cornea contained the proenzyme form of gelatinase A, whereas the peripheral cornea contained both the proenzyme form and the active form of gelatinase A. CONCLUSIONS: Fibroblasts located in the central cornea have a significantly higher rate of proliferation and ECM production than those of the peripheral cornea, whereas the presence of active gelatinase only in the peripheral cornea suggests higher gelatinolytic activity and ECM turnover in the periphery.


This article has been cited by other articles:


Home page
 JCBHome page
S. Chakravarti, T. Magnuson, J. H. Lass, K. J. Jepsen, C. LaMantia, and H. Carroll
Lumican Regulates Collagen Fibril Assembly: Skin Fragility and Corneal Opacity in the Absence of Lumican
J. Cell Biol., June 1, 1998; 141(5): 1277 - 1286.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by the Association for Research in Vision and Ophthalmology