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Investigative Ophthalmology & Visual Science, Vol 37, 2089-2098, Copyright © 1996 by Association for Research in Vision and Ophthalmology
ARTICLES AND REPORTS |
BA Berkowitz
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, USA.
PURPOSE: To test the hypothesis that breathing carbogen (95% O2-5% CO2) oxygenates the inner retina better than breathing 100% oxygen using an magnetic resonance imaging (MRI) method that noninvasively measures inner retinal oxygenation in normal adult and newborn rats. METHODS: Urethane-anesthetized adult and newborn (day 18) rats were studied. Sequential images were acquired in room air combined with either 100% oxygen or carbogen breathing. Normalized vitreous signal intensity changes were converted to oxygen tension changes (delta PO2) either on a pixel-by-pixel basis or in specific regions of interest. RESULTS: Systemic levels of hyperoxia during carbogen or 100% oxygen breathing were not significantly different (P > 0.05). In the adult rat, a significant difference (P = 0.017) was found in the preretinal vitreous delta PO2 during the breathing of either carbogen (130 +/- 9 mm Hg, mean +/- SEM; n = 5) or 100% oxygen (88 +/- 16 mm Hg; n = 5). Agreement was found between the MRI-determined delta PO2 values and literature oxygen microelectrodes data. In the newborn rat, significant differences (P < 0.05) in the preretinal vitreous delta PO2 were found during carbogen (164 +/- 23 mm Hg; n = 3) and oxygen breathing (91 +/- 8 mm Hg; n = 3). CONCLUSIONS: MRI delta PO2 mapping demonstrated for the first time that in the normal adult and newborn rat eye, carbogen breathing oxygenates the inner retina better than 100% oxygen breathing.
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S. J. Cringle and D.-Y. Yu Intraretinal Oxygenation and Oxygen Consumption in the Rabbit during Systemic Hyperoxia Invest. Ophthalmol. Vis. Sci., September 1, 2004; 45(9): 3223 - 3228. [Abstract] [Full Text] [PDF]
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R. Roberts, W. Zhang, Y. Ito, and B. A. Berkowitz Spatial Pattern and Temporal Evolution of Retinal Oxygenation Response in Oxygen-Induced Retinopathy Invest. Ophthalmol. Vis. Sci., December 1, 2003; 44(12): 5315 - 5320. [Abstract] [Full Text] [PDF]
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