IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stone, J.
Right arrow Articles by Keshet, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stone, J.
Right arrow Articles by Keshet, E.

Investigative Ophthalmology & Visual Science, Vol 37, 290-299, Copyright © 1996 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Roles of vascular endothelial growth factor and astrocyte degeneration in the genesis of retinopathy of prematurity

J Stone, T Chan-Ling, J Pe'er, A Itin, H Gnessin and E Keshet
Department of Molecular Biology, Hebrew University, Jerusalem.

PURPOSE: To assess the role of vascular endothelial growth factor (VEGF) in the feline model of retinopathy of prematurity (ROP). METHODS: Retinopathy of prematurity was induced in neonatal cats by raising them in an oxygen-enriched (70% to 80%) atmosphere for 4 days to suppress vessel formation and then returning them to room air for 3 to 27 days. In situ hybridization was used to detect the expression of VEGF and its high-affinity receptor, flk-1, in the retina of neonatal cats, and glial fibrillary acidic protein immunocytochemistry was used to assess astrocyte status. RESULTS: The expression of VEGF in the innermost layers of retina fell in hyperoxia and increased on return to room air. Vascular endothelial growth factor expression was transient; it was maximal where vessels were about to form, and it rapidly downregulated after vessels had formed. During the proliferative vasculopathy of ROP, VEGF expression was stronger than in the normally developing retina, and the astrocytes that normally express VEGF degenerated. After the degeneration of astrocytes, VEGF was expressed by neurones of the ganglion cell layer. flk-1 was expressed by intraretinal and preretinal vessels. Supplemental oxygen therapy reduced or eliminated the overexpression of VEGF expression, astrocyte degeneration, and formation of preretinal vessels. CONCLUSIONS: Regulation of VEGF by tissue oxygen mediates the inhibition of vessel growth during hyperoxia and the subsequent proliferative vasculopathy. Degeneration of retinal astrocytes creates conditions for the growth of preretinal vessels.


This article has been cited by other articles:


Home page
 IOVSHome page
L. E. H. Smith
Through The Eyes of a Child: Understanding Retinopathy through ROP The Friedenwald Lecture
Invest. Ophthalmol. Vis. Sci., December 1, 2008; 49(12): 5177 - 5182.
[Full Text] [PDF]

Home page
 Br. J. Ophthalmol.Home page
C Pieh, H Agostini, C Buschbeck, M Kruger, J Schulte-Monting, U Zirrgiebel, J Drevs, and W A Lagreze
VEGF-A, VEGFR-1, VEGFR-2 and Tie2 levels in plasma of premature infants: relationship to retinopathy of prematurity
Br. J. Ophthalmol., May 1, 2008; 92(5): 689 - 693.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. D. Akula, R. M. Hansen, M. E. Martinez-Perez, and A. B. Fulton
Rod Photoreceptor Function Predicts Blood Vessel Abnormality in Retinopathy of Prematurity
Invest. Ophthalmol. Vis. Sci., September 1, 2007; 48(9): 4351 - 4359.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Hughes, T. Gardiner, L. Baxter, and T. Chan-Ling
Changes in Pericytes and Smooth Muscle Cells in the Kitten Model of Retinopathy of Prematurity: Implications for Plus Disease
Invest. Ophthalmol. Vis. Sci., March 1, 2007; 48(3): 1368 - 1379.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
T. E. de Gooyer, K. A. Stevenson, P. Humphries, D. A. C. Simpson, T. M. Curtis, T. A. Gardiner, and A. W. Stitt
Rod Photoreceptor Loss in Rho-/- Mice Reduces Retinal Hypoxia and Hypoxia-Regulated Gene Expression
Invest. Ophthalmol. Vis. Sci., December 1, 2006; 47(12): 5553 - 5560.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. Liu, J. D. Akula, C. Falk, R. M. Hansen, and A. B. Fulton
The Retinal Vasculature and Function of the Neural Retina in a Rat Model of Retinopathy of Prematurity.
Invest. Ophthalmol. Vis. Sci., June 1, 2006; 47(6): 2639 - 2647.
[Abstract] [Full Text] [PDF]

Home page
 Integr Cancer TherHome page
C. R. Pradeep, E. S. Sunila, and G. Kuttan
Expression of Vascular Endothelial Growth Factor (VEGF) and VEGF Receptors in Tumor Angiogenesis and Malignancies
Integr Cancer Ther, December 1, 2005; 4(4): 315 - 321.
[Abstract] [PDF]

Home page
 IOVSHome page
H. Mechoulam and E. A. Pierce
Expression and Activation of STAT3 in Ischemia-Induced Retinopathy
Invest. Ophthalmol. Vis. Sci., December 1, 2005; 46(12): 4409 - 4416.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. H. Yoo, H.-J. Hyun, J.-Y. Koh, and Y. H. Yoon
Riluzole Inhibits VEGF-Induced Endothelial Cell Proliferation In Vitro and Hyperoxia-Induced Abnormal Vessel Formation In Vivo
Invest. Ophthalmol. Vis. Sci., December 1, 2005; 46(12): 4780 - 4787.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Economopoulou, K. Bdeir, D. B. Cines, F. Fogt, Y. Bdeir, J. Lubkowski, W. Lu, K. T. Preissner, H.-P. Hammes, and T. Chavakis
Inhibition of pathologic retinal neovascularization by {alpha}-defensins
Blood, December 1, 2005; 106(12): 3831 - 3838.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
K. A. Rezai, D. Eliott, P. J. Ferrone, and R. W. Kim
Near Confluent Laser Photocoagulation for the Treatment of Threshold Retinopathy of Prematurity
Arch Ophthalmol, May 1, 2005; 123(5): 621 - 626.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
P. van Wijngaarden, D. J. Coster, H. M. Brereton, I. L. Gibbins, and K. A. Williams
Strain-Dependent Differences in Oxygen-Induced Retinopathy in the Inbred Rat
Invest. Ophthalmol. Vis. Sci., April 1, 2005; 46(4): 1445 - 1452.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. F. Sun, T. Phung, I. Shiojima, T. Felske, J. N. Upalakalin, D. Feng, T. Kornaga, T. Dor, A. M. Dvorak, K. Walsh, et al.
Microvascular patterning is controlled by fine-tuning the Akt signal
PNAS, January 4, 2005; 102(1): 128 - 133.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
T. Chan-Ling, M. P. Page, T. Gardiner, L. Baxter, E. Rosinova, and S. Hughes
Desmin Ensheathment Ratio as an Indicator of Vessel Stability: Evidence in Normal Development and in Retinopathy of Prematurity
Am. J. Pathol., October 1, 2004; 165(4): 1301 - 1313.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. V. Ohlmann, E. Adamek, A. Ohlmann, and E. Lutjen-Drecoll
Norrie Gene Product Is Necessary for Regression of Hyaloid Vessels
Invest. Ophthalmol. Vis. Sci., July 1, 2004; 45(7): 2384 - 2390.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
J. L. Wilkinson-Berka, S. Babic, T. de Gooyer, A. W. Stitt, K. Jaworski, L. G. T. Ong, D. J. Kelly, and R. E. Gilbert
Inhibition of Platelet-Derived Growth Factor Promotes Pericyte Loss and Angiogenesis in Ischemic Retinopathy
Am. J. Pathol., April 1, 2004; 164(4): 1263 - 1273.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S.-C. Shih, M. Ju, N. Liu, J.-R. Mo, J. J. Ney, and L. E. H. Smith
Transforming growth factor {beta}1 induction of vascular endothelial growth factor receptor 1: Mechanism of pericyte-induced vascular survival in vivo
PNAS, December 23, 2003; 100(26): 15859 - 15864.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
S. Ishida, T. Usui, K. Yamashiro, Y. Kaji, S. Amano, Y. Ogura, T. Hida, Y. Oguchi, J. Ambati, J. W. Miller, et al.
VEGF164-mediated Inflammation Is Required for Pathological, but Not Physiological, Ischemia-induced Retinal Neovascularization
J. Exp. Med., August 4, 2003; 198(3): 483 - 489.
[Abstract] [Full Text] [PDF]

Home page
 Br. J. Ophthalmol.Home page
P E North, D C Anthony, T L Young, M Waner, H H Brown, and M C Brodsky
Retinal neovascular markers in retinopathy of prematurity: aetiological implications
Br. J. Ophthalmol., March 1, 2003; 87(3): 275 - 278.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. J. Sohn, B. K. Sarvis, D. Cado, and A. Winoto
ERK5 MAPK Regulates Embryonic Angiogenesis and Acts as a Hypoxia-sensitive Repressor of Vascular Endothelial Growth Factor Expression
J. Biol. Chem., November 1, 2002; 277(45): 43344 - 43351.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
W. Schacke, K.-F. Beck, J. Pfeilschifter, F. Koch, and L.-O. Hattenbach
Modulation of Tissue Plasminogen Activator and Plasminogen Activator Inhibitor-1 by Transforming Growth Factor-{beta} in Human Retinal Glial Cells
Invest. Ophthalmol. Vis. Sci., August 1, 2002; 43(8): 2799 - 2805.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
N. K. Ozaki, K. D. Beharry, K. C. Nishihara, Y. Akmal, J. G. Ang, R. Sheikh, and H. D. Modanlou
Regulation of Retinal Vascular Endothelial Growth Factor and Receptors in Rabbits Exposed to Hyperoxia
Invest. Ophthalmol. Vis. Sci., May 1, 2002; 43(5): 1546 - 1557.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
E. J. Duh, H. S. Yang, I. Suzuma, M. Miyagi, E. Youngman, K. Mori, M. Katai, L. Yan, K. Suzuma, K. West, et al.
Pigment Epithelium-Derived Factor Suppresses Ischemia-Induced Retinal Neovascularization and VEGF-Induced Migration and Growth
Invest. Ophthalmol. Vis. Sci., March 1, 2002; 43(3): 821 - 829.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
D. S. McLeod, M. Taomoto, J. Cao, Z. Zhu, L. Witte, and G. A. Lutty
Localization of VEGF Receptor-2 (KDR/Flk-1) and Effects of Blocking It in Oxygen-Induced Retinopathy
Invest. Ophthalmol. Vis. Sci., February 1, 2002; 43(2): 474 - 482.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
X. Gu, S. Samuel, M. El-shabrawey, R. B. Caldwell, M. Bartoli, D. M. Marcus, and S. E. Brooks
Effects of Sustained Hyperoxia on Revascularization in Experimental Retinopathy of Prematurity
Invest. Ophthalmol. Vis. Sci., February 1, 2002; 43(2): 496 - 502.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
N.W. GALE, G. THURSTON, S. DAVIS, S.J. WIEGAND, J. HOLASH, J.S. RUDGE, and G.D. YANCOPOULOS
Complementary and Coordinated Roles of the VEGFs and Angiopoietins during Normal and Pathologic Vascular Formation
Cold Spring Harb Symp Quant Biol, January 1, 2002; 67(0): 267 - 274.
[Abstract] [PDF]

Home page
 IOVSHome page
T. A. Drixler, I. H. M. B. Rinkes, E. D. Ritchie, F. W. Treffers, T. J. M. V. van Vroonhoven, M. F. B. G. Gebbink, and E. E. Voest
Angiostatin Inhibits Pathological but Not Physiological Retinal Angiogenesis
Invest. Ophthalmol. Vis. Sci., December 1, 2001; 42(13): 3325 - 3330.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
I. Nunes, R. D. Higgins, L. Zanetta, P. Shamamian, and S. P. Goff
c-Abl Is Required for the Development of Hyperoxia-induced Retinopathy
J. Exp. Med., June 18, 2001; 193(12): 1383 - 1392.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Stellmach, S. E. Crawford, W. Zhou, and N. Bouck
Prevention of ischemia-induced retinopathy by the natural ocular antiangiogenic agent pigment epithelium-derived factor
PNAS, January 23, 2001; (2001) 31252398.
[Abstract] [Full Text]

Home page
 Br. J. Ophthalmol.Home page
R. Axer-Siegel, M. Snir, D. Cotlear, A. Maayan, R. Frilling, I. Rosenbaltt, D. Weinberger, I. Kremer, and L. Sirota
Diode laser treatment of posterior retinopathy of prematurity
Br. J. Ophthalmol., December 1, 2000; 84(12): 1383 - 1386.
[Abstract] [Full Text]

Home page
 IOVSHome page
J. M. Provis, T. Sandercoe, and A. E. Hendrickson
Astrocytes and Blood Vessels Define the Foveal Rim during Primate Retinal Development
Invest. Ophthalmol. Vis. Sci., September 1, 2000; 41(10): 2827 - 2836.
[Abstract] [Full Text]

Home page
 Am. J. Pathol.Home page
K. Lashkari, T. Hirose, J. Yazdany, J. W. McMeel, A. Kazlauskas, and N. Rahimi
Vascular Endothelial Growth Factor and Hepatocyte Growth Factor Levels Are Differentially Elevated in Patients with Advanced Retinopathy of Prematurity
Am. J. Pathol., April 1, 2000; 156(4): 1337 - 1344.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
G. Neufeld, T. Cohen, S. Gengrinovitch, and Z. Poltorak
Vascular endothelial growth factor (VEGF) and its receptors
FASEB J, January 1, 1999; 13(1): 9 - 22.
[Abstract] [Full Text]

Home page
 Am. J. Pathol.Home page
H. Ozaki, N. Okamoto, S. Ortega, M. Chang, K. Ozaki, S. Sadda, M. A. Vinores, N. Derevjanik, D. J. Zack, C. Basilico, et al.
Basic Fibroblast Growth Factor Is Neither Necessary nor Sufficient for the Development of Retinal Neovascularization
Am. J. Pathol., September 1, 1998; 153(3): 757 - 765.
[Abstract] [Full Text] [PDF]

Home page
 PediatricsHome page
M. Mittal, R. Dhanireddy, and R. D. Higgins
Candida Sepsis and Association With Retinopathy of Prematurity
Pediatrics, April 1, 1998; 101(4): 654 - 657.
[Abstract] [Full Text] [PDF]

Home page
 Br. J. Ophthalmol.Home page
R. O SCHLINGEMANN and V. W M VAN HINSBERGH
Role of vascular permeability factor/vascular endothelial growth factor in eye disease
Br. J. Ophthalmol., June 1, 1997; 81(6): 501 - 512.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Stellmach, S. E. Crawford, W. Zhou, and N. Bouck
Prevention of ischemia-induced retinopathy by the natural ocular antiangiogenic agent pigment epithelium-derived factor
PNAS, February 27, 2001; 98(5): 2593 - 2597.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by the Association for Research in Vision and Ophthalmology