IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kurtz, R. M.
Right arrow Articles by Elner, S. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kurtz, R. M.
Right arrow Articles by Elner, S. G.

Investigative Ophthalmology & Visual Science, Vol 38, 436-445, Copyright © 1997 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Dexamethasone and cyclosporin A modulation of human retinal pigment epithelial cell monocyte chemotactic protein-1 and interleukin-8

RM Kurtz, VM Elner, ZM Bian, RM Strieter, SL Kunkel and SG Elner
Department of Ophthalmology (Kellogg Eye Center), University of Michigan, Ann Arbor 48105, USA.

PURPOSE: To examine the modulation of interleukin-1 beta (IL-1 beta)- and tumor necrosis factor-alpha (TNF-alpha)-stimulated monocyte chemotactic protein-1 (MCP-1) and interleukin-8 (IL-8) secretion and transcription in human retinal pigment epithelial (HRPE) cells by dexamethasone (DEX) and cyclosporin A (CSA). METHODS: Cultured HRPE cells were stimulated with IL-1 beta (0.2 to 20 ng/ml) or TNF-alpha (0.2 to 20 ng/ml) for 8 or 24 hours without (control) and with DEX (10(- 8) to 10(-6) M) or with CSA (0.3 to 30 ng/ml). Secreted levels of HRPE MCP-1 and IL-8 were measured in the media using enzyme-linked immunosorbent assay (ELISA). Both MCP-1 and IL-8 mRNA were analyzed by Northern blot. RESULTS: Although DEX (10(-8) to 10(-6) M) inhibited IL- 1 beta-stimulated MCP-1 and IL-8 production, it did not inhibit TNF- alpha-stimulated chemokine secretion. In contrast, CSA significantly inhibited TNF-alpha-stimulated, but not IL-1 beta-stimulated, HRPE MCP- 1 and IL-8 secretion. Both DEX and CSA inhibitions showed dose dependence. Northern blot analysis of HRPE steady state MCP-1 and IL-8 mRNA corroborated the ELISA measurements of secreted MCP-1 and IL-8. CONCLUSIONS: Although DEX and CSA inhibit HRPE MCP-1 and IL-8 secretion, this is dependent on whether the inducing inflammatory mediator is IL-1 beta or TNF-alpha. IL-1 beta-induced chemokine secretion is sensitive to DEX, whereas MCP-1 and IL-8 induced by TNF- alpha are inhibited by CSA. This information may be useful in explaining in vivo observations and in suggesting targeted clinical treatments and combinations of immunosuppressive agents.


This article has been cited by other articles:


Home page
 Br. J. Ophthalmol.Home page
A. Yoshida, S. G Elner, Z.-M. Bian, and V. M Elner
Induction of interleukin-8 in human retinal pigment epithelial cells after denuding injury
Br. J. Ophthalmol., July 1, 2001; 85(7): 872 - 876.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
A. Yoshida, S. Yoshida, Y. Hata, A. K. Khalil, T. Ishibashi, and H. Inomata
The Role of NF-{kappa}B in Retinal Neovascularization in the Rat: Possible Involvement of Cytokine-induced Neutrophil Chemoattractant (CINC), a Member of the Interleukin-8 Family
J. Histochem. Cytochem., April 1, 1998; 46(4): 429 - 436.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the Association for Research in Vision and Ophthalmology