IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nishi, O.
Right arrow Articles by Shirasawa, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nishi, O.
Right arrow Articles by Shirasawa, E.

Investigative Ophthalmology & Visual Science, Vol 38, 579-585, Copyright © 1997 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Detection of cell adhesion molecules in lens epithelial cells of human cataracts

O Nishi, K Nishi, T Akaishi and E Shirasawa
Nishi Eye Hospital, Osaka, Japan.

PURPOSE: To detect the expression of cell adhesion molecules (CAMs), including beta integrins, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1, leukocyte-endothelial cell adhesion molecule-2, E-cadherin, and CD44, in cultured lens epithelial cells (LECs) of human cataracts. To show that LECs attach to cells or extracellular matrix components by the detected adhesion molecules. METHODS: A circular section of the anterior capsule with attached LECs obtained by anterior capsulotomy during cataract surgery was cultured in a well of an eight-chamber slide. The LEC immediately after surgery and the cell outgrowth beyond the capsular margin at 2 weeks of culture were observed after the culture was stained immunohistochemically. Functional assays of LEC growth on collagen- or laminin-coated plates were performed in the presence and absence of the antibody blocking the detected adhesion molecules. RESULTS: beta 1 integrin, ICAM-1, and CD44 were detected in both the original specimens and the cultured cells. When the antihuman anti-beta 1 integrin monoclonal antibody (mAb), anti- ICAM-1 mAb, or anti-CD44 mAb was added at 10 micrograms/ml to the incubation medium, LEC migration and proliferation were inhibited significantly on the collagen- or laminin-coated plates. When the mAb blocking these three CAMs were added each at 1 microgram/ml, LEC proliferation also were inhibited. CONCLUSIONS: beta 1 integrin, ICAM-1 and CD44 are all involved in LEC attachment and growth on collagen and laminin in vitro. It can be assumed that these CAMs are involved in adhesion of LECs to extracellular matrix components of the lens capsule. Understanding the characteristics of the adhesion molecules in LEC may lead to the development of a new approach to inhibit secondary cataract formation.


This article has been cited by other articles:


Home page
 Br. J. Ophthalmol.Home page
S M McLean, M R K Mathew, J B Kelly, S B Murray, H G B Bennett, L A Webb, L Esakowitz, and J S McLean
Detection of integrins in human cataract lens epithelial cells and two mammalian lens epithelial cell lines
Br. J. Ophthalmol., November 1, 2005; 89(11): 1506 - 1509.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
B. K. Straub, J. Boda, C. Kuhn, M. Schnoelzer, U. Korf, T. Kempf, H. Spring, M. Hatzfeld, and W. W. Franke
A novel cell-cell junction system: the cortex adhaerens mosaic of lens fiber cells
J. Cell Sci., December 15, 2003; 116(24): 4985 - 4995.
[Abstract] [Full Text] [PDF]

Home page
 Br. J. Ophthalmol.Home page
W R MEACOCK, D J SPALTON, and M R STANFORD
Role of cytokines in the pathogenesis of posterior capsule opacification
Br. J. Ophthalmol., March 1, 2000; 84(3): 332 - 336.
[Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the Association for Research in Vision and Ophthalmology