| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Investigative Ophthalmology & Visual Science, Vol 38, 1293-1303, Copyright © 1997 by Association for Research in Vision and Ophthalmology
ARTICLES AND REPORTS |
T Belecky-Adams, S Tomarev, HS Li, L Ploder, RR McInnes, O Sundin and R Adler
Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287-9257, USA.
PURPOSE: To study the expression patterns of the homeobox genes Pax-6, Prox 1, and Chx10 during chick retinal development in vivo and in vitro. METHODS: Sections of paraformaldehyde-fixed, paraffin-embedded eyes were obtained at a range of developmental stages. In situ hybridization was carried out on tissue sections using digoxigenin- labeled sense and antisense RNA probes that recognize chicken Pax-6 and Prox 1 (whose sequences were already available), and chicken Chx10 (which was cloned and sequenced as part of this study). Selected developmental stages were also studied by immunocytochemistry with antibodies against Pax-6 and Prox 1, and by Northern blot analysis using 32P-labeled probes. RESULTS: Until embryonic day (ED) 5, in situ hybridization shows widespread, diffuse distribution of all three genes. Between ED 6 and ED 8, however, they acquire distinct, topographically specific patterns of expression. The Prox 1 signal is predominantly expressed in the prospective horizontal cell layer of the neuroepithelium, decreases vitreally, and is absent from ganglion cells and the prospective photoreceptor layer. Pax-6 is strongly expressed only in the prospective ganglion-cell and amacrine-cell regions at the same stages, and is not detected in prospective photoreceptors. Chx10 expression becomes concentrated in the future bipolar-cell region of the inner nuclear layer. Similar patterns are maintained by ED 15 through ED 18, after cell differentiation has taken place. Pax-6 and Prox 1 immunoreactive materials showed nuclear localization and a pattern of laminar distribution equivalent to that seen by in situ hybridization. CONCLUSIONS: These results suggest that the differentiated fate of retinal precursor cells may be influenced by Pax- 6, Prox 1, or Chx10, this hypothesis is now being tested using dissociated chick embryo retinal cell cultures.
This article has been cited by other articles:
|
|
 |
|
  T. Haynes, C. Gutierrez, J.-C. Aycinena, P. A. Tsonis, and K. Del Rio-Tsonis BMP signaling mediates stem/progenitor cell-induced retina regeneration PNAS, December 18, 2007; 104(51): 20380 - 20385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Li, D. Goldowitz, and D. J. Swanson The Requirement of Pax6 for Postnatal Eye Development: Evidence from Experimental Mouse Chimeras Invest. Ophthalmol. Vis. Sci., July 1, 2007; 48(7): 3292 - 3300. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Osakada, S. Ooto, T. Akagi, M. Mandai, A. Akaike, and M. Takahashi Wnt Signaling Promotes Regeneration in the Retina of Adult Mammals J. Neurosci., April 11, 2007; 27(15): 4210 - 4219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Xu, D. D. Sta. Iglesia, J. L. Kielczewski, D. F. Valenta, M. E. Pease, D. J. Zack, and H. A. Quigley Characteristics of Progenitor Cells Derived from Adult Ciliary Body in Mouse, Rat, and Human Eyes Invest. Ophthalmol. Vis. Sci., April 1, 2007; 48(4): 1674 - 1682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Liu, S. Thurig, O. Mohamed, D. Dufort, and V. A. Wallace Mapping Canonical Wnt Signaling in the Developing and Adult Retina Invest. Ophthalmol. Vis. Sci., November 1, 2006; 47(11): 5088 - 5097. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tanabe, Y. Takahashi, Y. Sato, K. Kawakami, M. Takeichi, and S. Nakagawa Cadherin is required for dendritic morphogenesis and synaptic terminal organization of retinal horizontal cells Development, October 15, 2006; 133(20): 4085 - 4096. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Shin, M. Min, F. Larrieu-Lahargue, X. Canron, R. Kunstfeld, L. Nguyen, J. E. Henderson, A. Bikfalvi, M. Detmar, and Y.-K. Hong Prox1 Promotes Lineage-specific Expression of Fibroblast Growth Factor (FGF) Receptor-3 in Lymphatic Endothelium: A Role for FGF Signaling in Lymphangiogenesis Mol. Biol. Cell, February 1, 2006; 17(2): 576 - 584. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. Paraoanu, J. B. Mocko, M. Becker-Roeck, J. Smidek-Huhn, and P. G. Layer Exposure to Diazinon Alters In Vitro Retinogenesis: Retinospheroid Morphology, Development of Chicken Retinal Cell Types, and Gene Expression Toxicol. Sci., January 1, 2006; 89(1): 314 - 324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Kubo, M. Takeichi, and S. Nakagawa Wnt2b inhibits differentiation of retinal progenitor cells in the absence of Notch activity by downregulating the expression of proneural genes Development, June 15, 2005; 132(12): 2759 - 2770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-A. Bruun, E. I. S. Thomassen, K. Kristiansen, G. Tylden, T. Holm, I. Mikkola, G. Bjorkoy, and T. Johansen The third helix of the homeodomain of paired class homeodomain proteins acts as a recognition helix both for DNA and protein interactions Nucleic Acids Res., May 10, 2005; 33(8): 2661 - 2675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. de Melo, G. Du, M. Fonseca, L.-A. Gillespie, W. J. Turk, J. L. R. Rubenstein, and D. D. Eisenstat Dlx1 and Dlx2 function is necessary for terminal differentiation and survival of late-born retinal ganglion cells in the developing mouse retina Development, January 15, 2005; 132(2): 311 - 322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Rowan, C.-M. A. Chen, T. L. Young, D. E. Fisher, and C. L. Cepko Transdifferentiation of the retina into pigmented cells in ocular retardation mice defines a new function of the homeodomain gene Chx10 Development, October 15, 2004; 131(20): 5139 - 5152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Spence, M. Madhavan, J. D. Ewing, D. K. Jones, B. M. Lehman, and K. Del Rio-Tsonis The hedgehog pathway is a modulator of retina regeneration Development, September 15, 2004; 131(18): 4607 - 4621. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Cui, S. I. Tomarev, J. Piatigorsky, A. B. Chepelinsky, and M. K. Duncan Mafs, Prox1, and Pax6 Can Regulate Chicken {beta}B1-Crystallin Gene Expression J. Biol. Chem., March 19, 2004; 279(12): 11088 - 11095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-H. D. Edqvist and F. Hallbook Newborn horizontal cells migrate bi-directionally across the neuroepithelium during retinal development Development, March 15, 2004; 131(6): 1343 - 1351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Rutherford, N. Dhomen, H. K. Smith, and J. C. Sowden Delayed Expression of the Crx Gene and Photoreceptor Development in the Chx10-Deficient Retina Invest. Ophthalmol. Vis. Sci., February 1, 2004; 45(2): 375 - 384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gonzalez-Estevez, T. Momose, W. J. Gehring, and E. Salo Transgenic planarian lines obtained by electroporation using transposon-derived vectors and an eye-specific GFP marker PNAS, November 25, 2003; 100(24): 14046 - 14051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rothermel and P. G. Layer GDNF Regulates Chicken Rod Photoreceptor Development and Survival in Reaggregated Histotypic Retinal Spheres Invest. Ophthalmol. Vis. Sci., May 1, 2003; 44(5): 2221 - 2228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Green, J. L. Stubbs, and E. M. Levine Genetic rescue of cell number in a mouse model of microphthalmia: interactions between Chx10 and G1-phase cell cycle regulators Development, February 1, 2003; 130(3): 539 - 552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Kubo, M. Takeichi, and S. Nakagawa Wnt2b controls retinal cell differentiation at the ciliary marginal zone Development, February 1, 2003; 130(3): 587 - 598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Toy, J. S. Norton, S. R. Jibodh, and R. Adler Effects of Homeobox Genes on the Differentiation of Photoreceptor and Nonphotoreceptor Neurons Invest. Ophthalmol. Vis. Sci., November 1, 2002; 43(11): 3522 - 3529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Fischer, C. R. McGuire, B. D. Dierks, and T. A. Reh Insulin and Fibroblast Growth Factor 2 Activate a Neurogenic Program in Muller Glia of the Chicken Retina J. Neurosci., November 1, 2002; 22(21): 9387 - 9398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-M. A. Chen and C. L. Cepko The chicken RaxL gene plays a role in the initiation of photoreceptor differentiation Development, January 12, 2002; 129(23): 5363 - 5375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Adler and T. L. Belecky-Adams The role of bone morphogenetic proteins in the differentiation of the ventral optic cup Development, January 7, 2002; 129(13): 3161 - 3171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Fischer, B. D. Dierks, and T. A. Reh Exogenous growth factors induce the production of ganglion cells at the retinal margin Development, January 5, 2002; 129(9): 2283 - 2291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-M. Li, R.-T. Yan, and S.-Z. Wang Atrophy of Muller Glia and Photoreceptor Cells in Chick Retina Misexpressing cNSCL2 Invest. Ophthalmol. Vis. Sci., December 1, 2001; 42(13): 3103 - 3109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. Brown, S. Patel, J. Brzezinski, and T. Glaser Math5 is required for retinal ganglion cell and optic nerve formation Development, July 1, 2001; 128(13): 2497 - 2508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Fuhrmann, E. Levine, and T. Reh Extraocular mesenchyme patterns the optic vesicle during early eye development in the embryonic chick Development, January 11, 2000; 127(21): 4599 - 4609. [Abstract] [PDF]
|
|
|
|
|
|
S Winkler, F Loosli, T Henrich, Y Wakamatsu, and J Wittbrodt The conditional medaka mutation eyeless uncouples patterning and morphogenesis of the eye Development, January 5, 2000; 127(9): 1911 - 1919. [Abstract] [PDF]
|
|
|
|
|
|
K. Del Rio-Tsonis, S. I. Tomarev, and P. A. Tsonis Regulation of Prox 1 during Lens Regeneration Invest. Ophthalmol. Vis. Sci., August 1, 1999; 40(9): 2039 - 2045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nishina, S. Kohsaka, Y. Yamaguchi, H. Handa, A. Kawakami, H. Fujisawa, and N. Azuma PAX6 expression in the developing human eye Br. J. Ophthalmol., June 1, 1999; 83(6): 723 - 727. [Abstract] [Full Text]
|
|
|
|
|
|
P. Callaerts, A. M. Munoz-Marmol, S. Glardon, E. Castillo, H. Sun, W.-H. Li, W. J. Gehring, and E. Salo Isolation and expression of a Pax-6 gene in the regenerating and intact Planarian Dugesia(G)tigrina PNAS, January 19, 1999; 96(2): 558 - 563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Xu, X Zhang, S Heaney, A Yoon, A. Michelson, and R. Maas Regulation of Pax6 expression is conserved between mice and flies Development, January 1, 1999; 126(2): 383 - 395. [Abstract] [PDF]
|
|
|
|
|
|
J. Toy, J.-M. Yang, G. S. Leppert, and O. H. Sundin The Optx2 homeobox gene is expressed in early precursors of the eye and activates retina-specific genes PNAS, September 1, 1998; 95(18): 10643 - 10648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Brown, S Kanekar, M. Vetter, P. Tucker, D. Gemza, and T Glaser Math5 encodes a murine basic helix-loop-helix transcription factor expressed during early stages of retinal neurogenesis Development, January 12, 1998; 125(23): 4821 - 4833. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
