| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Investigative Ophthalmology & Visual Science, Vol 38, 1840-1847, Copyright © 1997 by Association for Research in Vision and Ophthalmology
ARTICLES AND REPORTS |
H Gao, X Qiao, F Hefti, JG Hollyfield and B Knusel
Andrus Gerontology Center, University of Southern California, Los Angeles 90089-0191, USA.
PURPOSE: Recent studies show that exogenous brain-derived neurotrophic factor (BDNF) can promote retinal ganglion cell survival in vivo and in vitro. BDNF is expressed by a subpopulation of cells in the ganglion cell layer (GCL). To investigate whether endogenous BDNF may play a role in neuronal protection after ganglion cell trauma, BDNF expression in the retina was examined after optic nerve (ON) injury. METHODS: The optic nerve in Sprague-Dawley rats was crushed intraorbitally posterior to the optic disc. For controls, the optic nerve on the opposite side in each animal was similarly exposed but was not crushed. After intervals of 6 hours to 6 weeks, eye tissues were processed for in situ hybridization, Northern blot, and RNase protection assay using radiolabeled rat riboprobes. RESULTS: After ON injury, BDNF expression was significantly elevated in cells restricted to the GCL, and more cells demonstrated expression of BDNF than were observed in the controls. Elevated BDNF expression was first observed at 24 hours, peaked at 48 hours, and declined to the basal level 2 weeks after ON injury. Quantitative analysis showed a fivefold to sixfold increase in the number of BDNF-positive cells and a 54% increase in BDNF signal intensity in individual cells in the GCL 48 hours after ON injury. In control retinas without ON injury, BDNF expression was localized to some cells in the GCL, as was observed in normal eyes without surgery. Northern blot and RNase protection assay demonstrated a 38% elevation in BDNF expression above control levels 48 hours after ON injury. CONCLUSIONS: These results indicate that cells in the GCL can upregulate gene expression of BDNF in response to ganglion cell axonal injury and suggest that endogenous BDNF may contribute to a natural neuroprotective process after ON injury.
This article has been cited by other articles:
|
|
 |
|
  A. J. Weber, C. D. Harman, and S. Viswanathan Effects of optic nerve injury, glaucoma, and neuroprotection on the survival, structure, and function of ganglion cells in the mammalian retina J. Physiol., September 15, 2008; 586(18): 4393 - 4400. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Weber and C. D. Harman BDNF Preserves the Dendritic Morphology of {alpha} and {beta} Ganglion Cells in the Cat Retina after Optic Nerve Injury Invest. Ophthalmol. Vis. Sci., June 1, 2008; 49(6): 2456 - 2463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Loeliger, T. Briscoe, and S. M. Rees BDNF Increases Survival of Retinal Dopaminergic Neurons after Prenatal Compromise Invest. Ophthalmol. Vis. Sci., March 1, 2008; 49(3): 1282 - 1289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Fan, X. Li, and N. G. F. Cooper CaMKII{alpha}B Mediates a Survival Response in Retinal Ganglion Cells Subjected to a Glutamate Stimulus Invest. Ophthalmol. Vis. Sci., August 1, 2007; 48(8): 3854 - 3863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Maeda, A. Sawada, M. Matsubara, Y. Nakai, A. Hara, and T. Yamamoto A Novel Neuroprotectant against Retinal Ganglion Cell Damage in a Glaucoma Model and an Optic Nerve Crush Model in the Rat Invest. Ophthalmol. Vis. Sci., March 1, 2004; 45(3): 851 - 856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Garcia, V. Forster, D. Hicks, and E. Vecino In Vivo Expression of Neurotrophins and Neurotrophin Receptors Is Conserved in Adult Porcine Retina In Vitro Invest. Ophthalmol. Vis. Sci., October 1, 2003; 44(10): 4532 - 4541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Takahata, H. Katsuki, T. Kume, D. Nakata, K. Ito, S. Muraoka, F. Yoneda, S. Kashii, Y. Honda, and A. Akaike Retinal Neuronal Death Induced by Intraocular Administration of a Nitric Oxide Donor and Its Rescue by Neurotrophic Factors in Rats Invest. Ophthalmol. Vis. Sci., April 1, 2003; 44(4): 1760 - 1766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Neal, J. Cunningham, I. Lever, S. Pezet, and M. Malcangio Mechanism by which Brain-Derived Neurotrophic Factor Increases Dopamine Release from the Rabbit Retina Invest. Ophthalmol. Vis. Sci., February 1, 2003; 44(2): 791 - 798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Harada, C. Harada, S. Kohsaka, E. Wada, K. Yoshida, S. Ohno, H. Mamada, K. Tanaka, L. F. Parada, and K. Wada Microglia-Muller Glia Cell Interactions Control Neurotrophic Factor Production during Light-Induced Retinal Degeneration J. Neurosci., November 1, 2002; 22(21): 9228 - 9236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Gao, X. Qiao, L. B. Cantor, and D. WuDunn Up-regulation of Brain-Derived Neurotrophic Factor Expression by Brimonidine in Rat Retinal Ganglion Cells Arch Ophthalmol, June 1, 2002; 120(6): 797 - 803. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cheng, P. Sapieha, P. Kittlerova, W. W. Hauswirth, and A. Di Polo TrkB Gene Transfer Protects Retinal Ganglion Cells from Axotomy-Induced Death In Vivo J. Neurosci., May 15, 2002; 22(10): 3977 - 3986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Wordinger, W. Lambert, R. Agarwal, M. Talati, and A. F. Clark Human Trabecular Meshwork Cells Secrete Neurotrophins and Express Neurotrophin Receptors (Trk) Invest. Ophthalmol. Vis. Sci., November 1, 2000; 41(12): 3833 - 3841. [Abstract] [Full Text]
|
|
|
|
|
|
A. Di Polo, L. Cheng, G. M. Bray, and A. J. Aguayo Colocalization of TrkB and Brain-Derived Neurotrophic Factor Proteins in Green-Red-Sensitive Cone Outer Segments Invest. Ophthalmol. Vis. Sci., November 1, 2000; 41(12): 4014 - 4021. [Abstract] [Full Text]
|
|
|
|
|
|
H. A. Quigley, S. J. McKinnon, D. J. Zack, M. E. Pease, L. A. Kerrigan–Baumrind, D. F. Kerrigan, and R. S. Mitchell Retrograde Axonal Transport of BDNF in Retinal Ganglion Cells Is Blocked by Acute IOP Elevation in Rats Invest. Ophthalmol. Vis. Sci., October 1, 2000; 41(11): 3460 - 3466. [Abstract] [Full Text]
|
|
|
|
|
|
N. Klocker, P. Kermer, J. H. Weishaupt, M. Labes, R. Ankerhold, and M. Bahr Brain-Derived Neurotrophic Factor-Mediated Neuroprotection of Adult Rat Retinal Ganglion Cells In Vivo Does Not Exclusively Depend on Phosphatidyl-Inositol-3'-Kinase/Protein Kinase B Signaling J. Neurosci., September 15, 2000; 20(18): 6962 - 6967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. Johnson, L. M. H. Deppmeier, S. K. F. Wentzien, I. Hsu, and J. C. Morrison Chronology of Optic Nerve Head and Retinal Responses to Elevated Intraocular Pressure Invest. Ophthalmol. Vis. Sci., February 1, 2000; 41(2): 431 - 442. [Abstract] [Full Text]
|
|
|
|
|
|
P. Kermer, N. Klocker, M. Labes, and M. Bahr Insulin-Like Growth Factor-I Protects Axotomized Rat Retinal Ganglion Cells from Secondary Death via PI3-K-Dependent Akt Phosphorylation and Inhibition of Caspase-3 In Vivo J. Neurosci., January 15, 2000; 20(2): 722 - 728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ikeda, H. Tanihara, Y. Honda, T. Tatsuno, H. Noguchi, and C. Nakayama BDNF Attenuates Retinal Cell Death Caused by Chemically Induced Hypoxia in Rats Invest. Ophthalmol. Vis. Sci., August 1, 1999; 40(9): 2130 - 2140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-H. Herzog and C. S. von Bartheld Contributions of the Optic Tectum and the Retina as Sources of Brain-Derived Neurotrophic Factor for Retinal Ganglion Cells in the Chick Embryo J. Neurosci., April 15, 1998; 18(8): 2891 - 2906. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
